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Invitrogen™ SARS-CoV-2 Spike Protein (RBD) (aa319-541), His Tag Recombinant Protein
HEK293 cells Recombinant Protein
Brand: Invitrogen™ RP87704
Additional Details : Weight : 0.01000kg
Description
It has recently been shown that SARS (severe acute respiratory syndrome) is caused by a human coronavirus. Human coronaviruses are the major cause of upper respiratory tract illness, such as the common cold, in humans. Coronaviruses are positive-stranded RNA viruses, featuring the largest viral RNA genomes known to date (27-31 kb). The first step in coronavirus infection is binding of the viral spike protein, a 139-kDa protein, to certain receptors on host cells. The spike protein is the main surface antigen of the coronavirus. The glycosylated spike protein (as well as the nucleocapsid protein) can be detected in infected cell culture supernatants with antisera from SARS patients.- Purity: > 95% by SDS-PAGE; > 95% by HPLC
- Endotoxin Concentration: < 0.1 EU/μg of the protein by LAL method.
- Protein Length/ AA Sequence: Arg319-Phe541 of SARS-COV-2(2019-nCoV) Spike RBD fused with a 6×His tag at the C-terminus.
- Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2 Spike RBD at 2 μg/mL (100 μL/well) can bind recombinant Human ACE2 with a linear range of 0.15-3.63 ng/mL.
- Molecular Weight: 25.9 kDa
- Animal Origin Free: Yes Storage and Reconstitution: Store the lyophilized protein at -20°C to -80°C for long term. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
Specifications
Control, ELISA, Functional Assay, Western Blot | |
25.9 kDa | |
100 μg | |
-20°Cor to +80°C if preferred | |
Coronavirus; COVID; Covid 19; COVID19; COVID-19; SARS; SARS Coronavirus; SARS CoV; SARS CoV2; SARS CoV-2; SARS Spike Protein; SARS-CoV-2 | |
Unconjugated | |
Lyophilized |
PBS with no preservative; pH 7.4 | |
SARS-COV-2 Spike Protein (S-RBD)(aa319-541), His Tag | |
HEK293 cells | |
RUO | |
Virus | |
Recombinant | |
>95% |