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SIK1 Recombinant Human Protein

SIK1 Recombinant Human Protein

Brand:  Invitrogen PV6446

UNSPSC : 12352202

Additional Details : Weight : 0.01000kg

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Product Code. 15479053

  • 9700.00 SEK / 100µg


Estimated Shipment date
from Supplier 30-08-2017
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Disclaimers:

For Research Use Only. Not for use in diagnostic procedures.


Description

Description

SIK1 is a protein kinase that is involved in regulating AMPK-related kinases. This recombinant human SIK1 (1-303) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

  • Validated in LanthaScreen™ Kinase Binding Assay
  • Protocols available for simple use and optimized performance

    SIK1 may mediate the physiological effects of LKB1, including its tumour suppressor function. SIK1 is also involved in signaling by various proteins like STRAD, NUAK1, NUAK2, BRSK1, BRSK2, QIK, QSK, SIK, MARK1, MARK2, MARK3, MARK4, and MELK that are related to AMPK. Activation of SIK1 by phosphorylation on thr322 can lead to an increase in the catalytic activity of sodium/potassium ATPase alpha subunit at the plasma membrane. This results in an increase in intracellular sodium in intact mammalian cells.

    Learn more about our kinase proteins >

    Related Links:
    LanthaScreen™ Kinase Activity Assay
    LanthaScreen™ Eu Kinase Binding Assay
  • Specifications

    Specifications

    NP_173354
    Pharma and biopharma, target and lead identification and validation
    61kDa
    7
    100μg
    For research use only. Not for use in diagnostic procedures
    SNF1LK, MSK, SIK1
    SIK1 was pre-diluted in enzyme dilution buffer and assayed in 5mM MOPS (pH 7.2), 2.5mM β-glycerol-phosphate, 5mM MgCl2, 0.4mM EDTA, 1mM EGTA, 0.05mM DTT, with 50μM ATP, trace [33P]-γ-ATP and 200μg/mL Amara synthetic peptide substrate (AMARAASAAALARRR) for 15 min.s at 30°C
    Baculovirus
    SIK1 Recombinant Human Protein
    Human
    0.1mg/mL
    150094
    SIK1 Recombinant Human Protein
    90% by SDS-PAGE
    –68 to –85°C
    1 Each
    SIK1 Recombinant Human Protein
    Kinase assay
    Amino acids 1-303
    155nmoles of phosphate transferred to Amara synthetic peptide substrate (AMARAASAAALARRR) per minute per mg of total protein at 30°C
    GST Tag