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TFPI Recombinant Human Protein, His Tag, Invitrogen™ Sino Biological™

This recombinant protein was expressed from a DNA sequence encoding the human TFPI (NP_006278.1) (Met 1-Lys 282) fused to a C-terminal polyhistidine tag

Brand:  Invitrogen 10564H08H50

Additional Details : Weight : 0.10000kg

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Product Code. 15246935

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Description

Description

Tissue Factor Pathway Inhibitor (Liporeotein-Associated Coagulation Inhibitor) (TFPI), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in protein studies such as enzymatic analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.
  • Activity: Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (Anaspec Cat. No.27114), the IC50 value is <0.4nM, as measured in 100μL reaction mixture containing 1.25ng trypsin (Sigma Cat. No.T4799), 10μM substrate, 50mM Tris, 10mM CaCl2, 0.15M NaCl, 0.05% Brij-35, pH 7.5
  • Formulation: Lyophilized in 140mM NaCl, 2.7mM KCl, 10mM Na2HPO4, 1.8mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80
  • Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

This protein is manufactured by Sino Biological Inc.

Specifications

Specifications

Protein Expression and Isolation, Enzyme and Protein Activity Assay, Protein Assay Control, Reference Standards and Accessories
50μg
<1 EU/μg
Tissue factor pathway inhibitor (liporeotein-associated coagulation inhibitor)
His Tag
Protease Inhibitors
Proteinase Inhibitor
30.6kDa
Store at -20°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.
EPI, LACI, TFI, TFPI1
TFPI
Mammalian
Lyophilized
>85% by SDS-PAGE
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For Research Use Only. Not for use in diagnostic procedures.