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SK-BR-3 (human breast adenocarcinoma) Whole cell lysate, Non-denatured; Abnova

Produktkod. 16079625
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Kvantitet:
200 μg
Förpackningsstorlek:
200µg
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16079625 200 μg 200µg
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Produktkod. 16079625 Leverantör Abnova Leverantörsnummer L050V2.200ug

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Beskrivning

  • Tissue: Breast
  • Host: Human
  • Lysis buffer: Modified RIPA lysis buffer: 50mM Tris-HCl pH 7.4, 150mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
  • Storage buffer: In modified RIPA lysis buffer

Western Blotting, Immunoprecipitation

Specifikationer

För användning med (applikation) Immunoprecipitation, Western Blot
Vävnad Breast
Beskrivning Whole cell lysate (non-denatured)
Lysisbuffert Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Beredningsmetod Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay).
Kvalitetskontrolltestning 12.5% SDS-PAGE Stained with Coomassie Blue.
Kvantitet 200 μg
Förvaringsbuffert In modified RIPA Lysis Buffer.
Värd art Human
Koncentration 5 mg/mL
Innehåll och lagring Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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