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Beskrivning
Slide-A-Lyzer Dialyskassetter (10K MWCO) underlättar enkel och effektiv borttagning av salter och små föroreningar från proteiner och andra makromolekyler med ett värde över 10 000 Dalton. Slide-A-Lyzer Dialyskassetter finns i fyra storlekar för att rymma provvolymer mellan 0,5 och 30 ml. Dessa enheter är enklare att använda än traditionella dialysrör och ger snabbare buffertutbyte och mer komplett provutvinning.
Slide-A-Lyzer cassettes can be used for a wide range of applications including low-molecular weight contaminant removal, buffer exchange, desalting, equilibrium dialysis, and sample concentration. Liquid samples are easily added and removed by penetrating the self-sealing gasket with a hypodermic needle attached to a syringe. No knots, clips, or caps are needed to seal the units and prevent leaking and sample-loss during the dialysis procedure.
Slide-A-Lyzer Dialysis Cassettes are constructed from two sheets of low-binding, regenerated-cellulose dialysis membrane that are hermetically sealed on either side of a silicone-like gasket inside an inert plastic frame. The membrane and cassette materials are compatible with most common laboratory chemicals and buffers.
Features of Slide-A-Lyzer Dialysis Cassettes:
- Color-coded—orange frame specifies cassettes with the 10,000 MW pore size membrane
- Four cassette sizes—select the cassette that best suits your sample volume
- Fast dialysis—flat cassette chamber with two membranes provides high surface area-to-volume ratio that maximizes diffusion rate compared to cylindrical dialysis tubing
- High recovery—rectangular cassette design maximizes recovery of entire sample volume via any one of the four corner injection ports
- Gamma-irradiated option—gamma-irradiated cassettes are available for applications requiring conditions that minimize the risk of sample contamination
Gamma-irradiated dialysis cassettes
Gamma-irradiated Slide-A-Lyzer Dialysis Cassettes are ideal for researchers culturing cells and microorganisms; purifying viruses, DNA, and RNA; or performing sample preparation for other applications requiring conditions that minimize the risk of sample contamination. The gamma-irradiated cassettes are treated with 20–36 kGy of gamma radiation, a dosage that has been determined to sterilize the units based on both trypticase soy broth medium for identifying total growth and fluid thioglycollate medium for identifying anaerobic growth. However, a confirmation of sterility is not provided for individual lots, as no further sterility testing is conducted post-irradiation.
Membrane specifications:
- Composition: regenerated cellulose synthesized by the viscose method
- Thickness: 0.9 to 1.2 mil (22.5 to 30 μm)
- Hydration time required before use: 2 minutes
- Glycerol content: 21%
- Sulfur content: 0.1–0.15%
- Heavy metals content: trace
Volume ranges:
- 0.5 mL = 0.1 to 0.5 mL
- 3 mL = 0.5 to 3 mL
- 12 mL = 3 to 12 mL
- 30 mL = 12 to 30 mL
Specifikationer
Specifikationer
| Innehåll och lagring | Store at room temperature |
| Reningsmål | Buffer Exchange, Protein |
| Membran | Cellulose Membrane, Dialysis |
| Formatera | Cassette |
| MWCO | 10 kDa |
| Produktlinje | Slide-A-Lyzer |
| Kvantitet | 10 Cassettes |
| Samplingsvolym (metrisk) | 0.5 mL |
| Produkttyp | Dialysis Cassettes |
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