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Beskrivning
pDSG-IBA vectors for MEXi mammalian expression system
- Small transient expression vector especially developed for the use in combination with the MEXi mammalian expression system.
- Designed for high-level stable and non-replicative transient expression in most mammalian hosts containing the following elements:
- Human cytomegalovirus (CMV) immediate-early promoter for high-level expression in a wide range of mammalian cells
- Episomal replication through Epstein Barr Virus replication origin (oriP) requires chromosomally expressed nuclear antigen encoded by EBNA-1
- Ampicillin resistance and ColE1 replication origin (pUC) for propagation in E. coliin a high plasmid copy number
Please note that cloning into pDSG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pDSG-IBA vectors with Esp3I, another option via a so-called Entry-Vector is possible.
Specifikationer
Specifikationer
| Konstitutivt eller inducerbart system | Constitutive |
| Markör Gene | Ampicillin |
| Promotor | CMV |
| Produkttyp | Vector |
| Urvalsagent (eukaryot) | Geneticin™ (G-418) |
| Innehåll och lagring | Recommended: 2°C to 8°C for frequent usage, -20°C for long-term storage |
| Protein Tag | His Tag (6x) |
| Primer | Forward |
| Kvantitet | 5 μg |
| Vektor | pDSG-IBA33 |
Produkttitel
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