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Thermo Scientific™ SMART Digest™ Dual Enzyme Columns for HDX-MS Analysis

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18078081 ˜20 μm
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Produktkod. 18078081 Leverantör Thermo Scientific™ Leverantörsnummer 60160110
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Beräknad leverans: 25-09-2026
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Beskrivning

Thermo Scientific™ SMART Digest™ Dual Enzyme Columns for HDX-MS analysis provide fast, reliable digestion of proteins for HDX-MS analysis, helping to provide the sequence characterization of biotherapeutics.

Thermo Scientific SMART Digest Dual Enzyme columns containing pepsin and protease XIII provide fast, reliable digestion of proteins for for HDX-MS analysis helping to provide the sequence characterization of biotherapeutics.

SMART Digest Dual Enzyme columns for HDX-MS analysis help to provide the characterization of biotherapeutics.

The combination of enzymes are complementary, creating many short, overlapping N-terminal peptides for a high degree of sequence coverage.

Pepsin cleaves proteins preferentially at carboxylic groups of aromatic amino acids such as phenylalanine, tryptophan and tyrosine. It will not cleave at bonds containing valine, alanine or glycine. The optimum pH range for pepsin activity is 1.0–3.0.

Proteinase XIII cleaves proteins preferentially at carboxylic groups of basic amino acids (arginine, lysine, histidine). Like pepsin, Proteinase XIII is active at low pH.

TRUSTED_SUSTAINABILITY

Specifikationer

Typ SMART Digest Pepsin/Protease XIII Dual Enzyme column
Beskrivning SMART Digest Pepsin/Protease XIII Dual Enzyme Column
Enzym Pepsin & Protease XIII
Klyvning Pepsin: Cleaves proteins preferentially at carboxylic groups of aromatic amino acids such as phenylalanine, tryptophan and tyrosine. The optimum pH range for pepsin activity is 1.0–3.0.

Protease XIII: Cleaves proteins preferentially at carboxylic groups of basic amino acids (arginine, lysine, histidine). Like pepsin, Proteinase XIII is active at low pH.
För användning med (applikation) HDX-MS analysis
Kolumnformat Immobilized enzyme
Diameter 2.1 mm
Flödeshastighet 10 to 500 μL/min
Max. Tryck 2500 psi (170 bar)
Längd 30 mm
Mobilfaskompatibilitet Phosphate buffer pH 4
Partikelstorlek ˜20 μm
pH 2 to 6
Porstorlek ° 1000 angstroms
Drifttemperaturområde 4 °C to 50 °C
Förvaringstemperatur RT for up to 2 weeks; 4 °C for extended period (refrigerate in MES pH 6)
Stationär fas Immobilized enzyme
Förpackningsmaterial Hydrophilic/coated DVB
Kolumntyp Immobilized enzyme in PEEK cartridge
Teknik BioLC
Stabilitet 1 year
Vikt 8 g
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