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Thermo Scientific™ Spectra™ Multicolor High Range Protein Ladder
A mixture of eight blue-, green- and orange-stained proteins (40 to 300kDa) for use as size standards for high MW proteins in gel electrophoresis and Western blotting.
Brand: Thermo Scientific™ 26625
2066.25 SEK valid until 2024-04-30
Use promo code "21741" to get your promotional price.
Additional Details : Weight : 1.36080kg
Description
Thermo Scientific™ Spectra Multicolor High Range Protein Ladder is a mixture of eight (8) blue-, green and orange-stained proteins (40 to 300kDa) for use as size standards for high MW proteins in gel electrophoresis and Western blotting.
This prestained protein MW marker is designed for monitoring the progress of SDS-polyacrylamide gel electrophoresis, for assessing transfer efficiency onto PVDF, nylon and nitrocellulose membranes, and for estimating the approximate size of separated proteins that have been made visible with gel stains or Western blot detection reagents. Three different chromophores (blue, orange, green) are bound to the different component proteins, producing a brightly colored ladder with an easy-to-remember pattern. The Spectra Multicolor High Range Protein Ladder is ready to use: no heating, further dilution or addition of a reducing agent is required.Highlights:
Size range – eight proteins spanning 40 to 300kDa
Multicolor – three different colors for unambiguous band-size assignment
Ready-to-use – supplied in a loading buffer for direct loading on gels; no need to boil
Sharp bands – color-coded bands of similar intensity for easy visualization
Quality tested – each lot evaluated by SDS-PAGE and Western blotting
Membrane-compatible – colored bands transfer to membranes for Western blotting
Includes:
Dye-stained proteins in 62.5mM Tris-H3PO4 (pH 7.5 at 25°C), 1mM EDTA, 2% SDS, 10mM DTT, 1mM NaN3 and 33% glycerol.
Recommended for:
Monitoring of protein migration during SDS-PAGE; Verifying Western transfer efficiency; Approximate sizing of proteins on SDS-polyacrylamide gels and Western blots; Locating a protein of interest for excision from an unstained preparative gel
Specifications
| Contents: two vials of 250 μL each Storage buffer: 62.5 mM Tris-H3PO4 (pH 7.5 at 25°C), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN3 and 33% glycerol Storage: Upon receipt store at -20°C |
|
| 8 | |
| Yes | |
| 40 to 300 kDa | |
| Bolt™ Bis-Tris Plus Gels, Novex™ Tricine Gels, Novex™ Tris-Glycine Gels, NuPAGE™ Bis-Tris Gels, NuPAGE™ Tris-Acetate Gels, SDS-PAGE Gels | |
| Spectra™ | |
| Approved for shipment on Wet or Dry Ice |
| Colorimetric, NIR Fluorescence (700 nm), RGB Fluorescence (555 nm) | |
| Protein Ladder | |
| Tris-acetate gels | |
| 3 colors: Blue, Orange, Green | |
| 300, 250, 180, 130, 100, 70, 50, 40 kDa | |
| 2 x 250 μL | |
| Western Blotting, SDS-PAGE |
For Research Use Only. Not for use in diagnostic procedures.