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SSEA1, clone: eBioMC-480 (MC-480), eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-8813-82

Additional Details : Weight : 0.01000kg

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Product Code. 15238097

  • 2035.00 SEK / 100µg


EU Stock 3
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Description

Description

Description: The eBioMC-480 (MC-480) antibody reacts with the stage-specific embryonic antigen-1 (SSEA-1), a carbohydrate epitope expressed upon the surface of early mouse embryos, murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES) and murine and human germ cells (EG). No immunoreactivity is evident with undifferentiated human EC and ES cells. Differentiation of human EC results in an increase in SSEA-1 expression, while in the mouse expression is diminished. Expression of the carbohydrate moiety is also found on mature human granulocytes (on CD15) and some monocytes. SSEA-1 is associated with cell adhesion, migration and differentiation.Applications Reported: This eBioMC-480 (MC-480) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining.Applications Tested: This eBioMC-480 (MC-480) antibody has been tested by flow cytometric analysis of the F9 cell line. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

SSEA1
Monoclonal
0.5mg/mL
PBS with 0.09% sodium azide; pH 7.2
stage-specific embryonic antigen 1, CD15, SSEA-1, SSEA1, ELFT, FCT3A, FUC-TIV, FUTIV, LeX
IgM
100μg
Store at 2-8°C.
Primary
HumanþMurine
Flow CytometryþImmunohistochemistryþImmunoprecipitation
eBioMC-480 (MC-480)
Unconjugated
Liquid
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
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