SSEA1, PerCP-eFluor™ 710, clone: eBioMC-480 (MC-480), eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 46-8813-42
Additional Details : Weight : 0.23750kg
DescriptionDescription: The eBioMC-480 (MC-480) antibody reacts with the stage-specific embryonic antigen-1 (SSEA-1), a carbohydrate epitope expressed upon the surface of early mouse embryos, murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES) and murine and human germ cells (EG). No immunoreactivity is evident with undifferentiated human EC and ES cells. Differentiation of human EC results in an increase in SSEA-1 expression, while in the mouse expression is diminished. Expression of the carbohydrate moiety is also found on mature human granulocytes (on CD15) and some monocytes. SSEA-1 is associated with cell adhesion, migration and differentiation.Applications Reported: This eBioMC-480 (MC-480) antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBioMC-480 (MC-480) antibody has been pre-titrated and tested by flow cytometric analysis of F9 cells. This can be used at 5 μL (0.125 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. PerCP-eFluor™ 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor™ 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative. Our testing indicates that PerCP-eFluor™ 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2|
|stage-specific embryonic antigen 1, CD15, SSEA-1, SSEA1, ELFT, FCT3A, FUC-TIV, FUTIV, LeX|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
For Research Use Only.