SSEA5 Monoclonal Antibody (8E11-SSEA5), eBioscience™, Invitrogen™

Description: The monoclonal antibody 8E11-SSEA5 recognizes human stage-specific embryonic antigen-5 (SSEA-5), an H type-1 glycan. SSEA-5 belongs to the SSEA family of glycoproteins and is highly expressed on pluripotent human stem cells including embryonic stem (ES) cells and induced pluripotent stem (iPS) cells. During embryonic development, SSEA-5 is expressed within the blastocyst on inner cell mass (ICM) cells that later give rise to ES cells. As ES and iPS cells differentiate, SSEA-5 expression is downregulated. The tumorigenic potential of contaminating stem cells in cell populations for transplantation is a hurdle to using this approach for disease therapy. The 8E11 antibody in combination with either CD9 and CD90 or CD50 and CD200 to sort populations has been shown to effectively separate teratoma-forming cells from stem cell-derived populations of differentiated cells.Applications Reported: This 8E11-SSEA5 antibody has been reported for use in flow cytometric analysis, western blotting, and immunocytochemistry.Applications Tested: This 8E11-SSEA5 antibody has been tested immunocytochemistry on unfixed human Ntera2 cells at less than or equal to 10 ug/mL. The 8E11-SSEA5 antibody has also been tested by flow cytometric analysis on NTERA-2 cells at less than or equal to 0.25 ug/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.