Läs mer
IBA Lifesciences StarGate pESG-IBA-vektorer för däggdjursuttryck
Handla allt IBA Lifesciences ProdukterBeschreibung
pESG-IBA is a small expression vector recommended for transient expression of proteins in cell lines providing SV40 large T-antigen for extrachromosomal replication with SV40 ori.
- NeoR for generation of stable cells lines.
- Cytomegalovirus immediate-early (CMV) promoter: high level constitutive expression
- SV40 ori: origin of replication for extrachromosomal replication driven by SV40 large T-antigen (as provided by HEK293T or COS-1, -3, -7 cells)
- ColE1 + Amp: for propagation in E. coli
- NeoR: neomycin resistance gene to generate stable cell lines
- BM40: secretion signal to transfer the expressed protein into the medium (not for all vectors available)
Please note that cloning into pESG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pESG-IBA vectors with Esp3I, another option via a so-called Entry-Vector is possible.since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pESG-IBA vectors with Esp3I, another option via a so-called Entry-Vector is possible.
Spezifikation
Spezifikation
| Formatera | Liquid |
| För användning med (utrustning) | StarGate™ System, Mammalian Host Cells |
| Inducerande medel | SV40 large T antigen (e.g. COS1 or COS7) |
| Inkluderar | Neomycin resistance gene |
| pH | 8 |
| Promotor | Human CMV Immediate-early |
| Produkttyp | Acceptor Vector for mammalian expression |
| Förvaringsbuffert | 20 μL TE buffer: 10 mm Tris-HCl, 1 mm EDTA / 5 μg |
| Innehåll och lagring | 2°C to 8°C for frequent usage, -20°C for long-term storage |
| Protein Tag | His Tag (6x) for N-terminal |
| Mehr anzeigen |
Bitte geben Sie uns Ihr Feedback zu den Produktinhalten, indem Sie das folgende Formular ausfüllen.