IBA Lifesciences StarGate pESG-IBA-vektorer för däggdjursuttryck

Designad för högnivåkonstitutivt uttryck av rekombinanta proteiner i ett brett spektrum av däggdjursvärdceller genom CMV-promotorn, med replikering i cellinjer som är latent infekterade med SV40 stort T-antigen.

pESG-IBA mammalian expression vectors

pESG-IBA is a small expression vector recommended for transient expression of proteins in cell lines providing SV40 large T-antigen for extrachromosomal replication with SV40 ori.

• NeoR for generation of stable cells lines.
• Cytomegalovirus immediate-early (CMV) promoter: high level constitutive expression
• SV40 ori: origin of replication for extrachromosomal replication driven by SV40 large T-antigen (as provided by HEK293T or COS-1, -3, -7 cells)
• ColE1 + Amp: for propagation in E. coli
• NeoR: neomycin resistance gene to generate stable cell lines
• BM40: secretion signal to transfer the expressed protein into the medium (not for all vectors available)

Please note that cloning into pESG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pESG-IBA vectors with Esp3I, another option via a so-called Entry-Vector is possible.since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pESG-IBA vectors with Esp3I, another option via a so-called Entry-Vector is possible.