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IBA Lifesciences StarGate pPSG-IBA Acceptor Vectors for E. coli Expression
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Vektor:
pPSG-IBA 105
pPSG-IBA 123
pPSG-IBA 164
pPSG-IBA 168
pPSG-IBA 23
pPSG-IBA 25
pPSG-IBA 3
pPSG-IBA 33
pPSG-IBA 5
pPSG-IBA 62
pPSG-IBA 63
pPSG-IBA 64
pPSG-IBA 65
pPSG-IBA wt1
pPSG-IBA103
pPSG-IBA143
pPSG-IBA145
pPSG-IBA162
pPSG-IBA167
pPSG-IBA35
pPSG-IBA43
pPSG-IBA45
Förpackningsstorlek:
5µg
Beskrivning
pPSG-IGA E. coli expression vectors:
pPSG-IBA vectors use the T7 promoter and T7 RNA polymerase for high-level transcription of the gene of interest.
- High-level expression in E. coli by bacteriophage T7 promoter
- High-level transcription by T7 RNA polymerase in BL21 strains
- High-level expression of non-toxic proteins
- Induction by IPTG
- Suitable for in vitro transcription/translation
StarGate System
IBA developed StarGate to provide a simple, fast and highly efficient cloning process for genes of interest (GOI) for use in functional expression, purification, detection, immobilization or separation of recombinant proteins:
- Minimal extra modification of the gene due to short combinatorial sites
- Inherent high-level cloning efficiency due to a directed reaction (no equilibrium)
- GOIs are cloned to obtain a Donor Vector; 2 or more genes can be combined using Fusion Cloning
- Genes are then transferred into Acceptor Vectors that provide optimal genetic surroundings
- The final expression vector, the Destination Vector, is then placed into the respective host
Specifikationer
Specifikationer
Formatera | Liquid |
För användning med (utrustning) | StarGate™ System, E. Coli BL21(DE3) |
Inducerande medel | E. coli host with T7 RNA polymerase gene (e.g. BL21(DE3)) |
pH | 8 |
Promotor | T7 |
Produkttyp | Acceptor Vector for E. coli expression |
Förvaringsbuffert | 20 μL TE buffer: 10 mm Tris-HCl, 1 mm EDTA / 5 μg |
Innehåll och lagring | 2°C to 8°C for frequent usage, -20°C for long-term storage |
Protein Tag | Strep-tag for N-terminal |
Koncentration | 250 ng/μL |
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Dokument
Product Information
Manuals
Manual StarGate 5-4205-001
Filstorlek: 2.3 MB
Manual StarGate-Direct-Transfer-Cloning 5-4205-001
Filstorlek: 1.7 MB
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Produkttitel
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