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IBA Lifesciences StarGate pYSG-IBA Vectors for Yeast Expression
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Beskrivning
The pYSG-IBA vector allows the high-level expression of proteins in yeast.
- The vector carries the copper inducible promoter (CUP1) for controlled high-level expression.
- The expressed recombinant protein will be localized in the cytoplasm.
- High-level expression in yeast with high copy number
- Favors correct protein folding and the production of soluble proteins - inclusion bodies rarely form
- Tightly regulated expression due to Cu++-inducible CUP1 promoter
- Ampicillin resistance gene and markers Leu2-d and URA3 in every vector
- Leu2-d auxotrophy marker for selection to increase plasmid copy number during expression
- URA3 auxotrophy marker for selection after transformation
Please note that cloning into pYSG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pYSG-IBA vectors with Esp3I, another option via a so-called Entry-Vector is possible.
Specifikationer
Specifikationer
| Formatera | Vätska |
| För användning med (utrustning) | StarGate™ System, Yeast Host Cells |
| Inducerande medel | Copper sulfate |
| Markör Gene | leu2-d and URA3 |
| pH | 8 |
| Promotor | CUP1 |
| Produkttyp | Acceptor Vector for yeast expression |
| Förvaringsbuffert | 20μ L TE-buffert: 10 mm Tris-HCl, 1 mm EDTA / 5μ g |
| Innehåll och lagring | 2 °C till °8 C för frekvent °användning, -20 C för långtidsförvaring |
| Protein Tag | Otaggad |
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