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IBA Solutions For Life Science StarGate™ Upstream Fusion Vector pNFUSE-IBA
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Vektor:
pNFUSE-IBA w/ IRES11
pNFUSE-IBA w/ LINK11
pNFUSE-IBA w/ LINK12
pNFUSE-IBA w/ SD11
Förpackningsstorlek:
5µg
Beskrivning
StarGate™ offers a simple cloning procedure for rapid and highly efficient subcloning of an arbitrary gene of interest (GOI) for use in functional expression, purification, detection, and immobilization or separation of recombinant proteins.
- Minimal extra modification of the gene due to short combinatorial sites
- Inherent high-level cloning efficiency due to a directed reaction (no equilibrium)
- Genes are cloned in a Donor Vector and then parallel-transfered into Acceptor Vectors that provide different genetic surroundings
- Easily connect two or more genes that are cloned in separate Donor Vectors using Fusion Vectors that are assembled by use of a one-tube reaction
- The GOI to be positioned upstream is transferred into a pNFUSE-IBA derivative, specifying the intergenic region (IR):
- IRES11: Provides internal ribosomal entry site (IRES) from encephalomyocarditis virus for separate cap-independent translation of downstream cistron in mammalian cells
- SD11: IR includes a Shine Dalgarno (SD) sequence for translation of downstream cistron at comparable level to the upstream cistron in bacterial cells
- LINK11: Genes of interest are fused by a sequence encoding the 10 amino acid linker GlySerGlyGlyGlySerGlyGlyGlySer
- LINK12: Genes of interest are fused by a sequence encoding the 15 amino acid linker GlySerGlyGlySerGlyGlyGlyGlySerGlyGlyGlyGlySer
- The GOIs to be positioned downstream are transferred into a pCFUSE-IBA11 vector (identical, irrespective of IR)
Specifikationer
Specifikationer
| Formatera | Solution, liquid |
| För användning med (utrustning) | Stargate™ system derivatives |
| Beskrivning | Resistance: ampicillin |
| Inkluderar | IR: Internal ribosomal entry site (IRES) from encephalomyocarditis virus |
| pH | 8 |
| Produkttyp | Fusion Vector |
| Förvaringsbuffert | 20μL TE buffer: 10mM Tris-HCl, 1mM EDTA per 5μL pCFUSE-IBA11 |
| Innehåll och lagring | 2°C to 8°C for frequent usage, -20°C for long-term storage |
| Koncentration | 250 ng/μL |
| Kvantitet | 5 μg |
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