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STRO-1 Monoclonal Antibody (STRO-1), eBioscience™, Invitrogen™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 14-6688-82
Additional Details : Weight : 0.09500kg
Description
Description: The monoclonal antibody STRO-1 recognizes STRO-1, a bone marrow (immature) mesenchymal cell surface protein. Pluripotent stromal cells can give rise to several lineages: osteoblasts and chondrocytes, which form bone, adipocytes or hematopoietic cells. STRO-1+ cells when co-transplanted with hematopoietic stem cells have been shown to reduce the occurrence of graft versus host disease. In addition, in vitro cultures of Stro-1+ cells along with CD34+ cells give rise to CFUs (colony forming units) composed of hematopoietic precursors, establishing a supporting role of these mesenchymal cells in blood cell development. The STRO-1 antibody has been reported to recognize both bovine and rat Stro-1 protein. Applications Reported: This STRO-1 antibody has been reported for use in flow cytometric analysis, immunocytochemistry, and immunohistochemical staining of frozen tissue sections. Applications Tested: This STRO-1 antibody has been tested by immunocytochemistry on methanol-fixed MG-63 cells and can be used at less than or equal to 20 μg/mL. The STRO-1 antibody has also been tested by flow cytometry on MG-63 cells and can be used at less than or equal to 0.5 μg/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered.
STRO-1 is a cell surface antigen expressed by stromal elements in human bone marrow. STRO-1 binds to approximately 10% of bone marrow mononuclear cells, greater than 95% of which are nucleated erythroid precursors, but does not react with committed progenitor cells (colony-forming unit granulocyte-macrophage (CFU-GM), erythroid bursts (BFU-E), and mixed colonies (CFU-Mix). From bone marrow cells, the frequency of fibroblast colony forming cells (CFU-F) is enriched approximately 100-fold in the STRO-1-positive/Glycophorin A-negative population than in the STRO-1-positive/Glycophorin A-positive population. When plated under long-term BM culture conditions, STRO-1-positive cells generate adherent cell layers containing multiple stromal cell types, including adipocytes, smooth muscle cells, and fibroblastic elements. A STRO-1-positive enriched subset of marrow cells is capable of differentiating into multiple mesenchymal lineages including hematopoiesis-supportive stromal cells with a vascular smooth muscle-like phenotype, adipocytes, osteoblasts and chondrocytes. STRO-1 is a valuable antibody for the identification, isolation and functional characterization of human bone marrow stromal cell precursors.Specifications
STRO-1 | |
Monoclonal | |
0.5 mg/mL | |
PBS with 0.09% sodium azide; pH 7.2 | |
Mouse | |
100 μg | |
Primary | |
4° C | |
Liquid |
Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Frozen) | |
STRO-1 | |
Unconjugated | |
STRO1 | |
Affinity chromatography | |
RUO | |
Bovine, Human, Rat | |
Antibody | |
IgM λ |
For Research Use Only.