T-bet, PE, clone: eBio4B10 (4B10), eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 12-5825-82
Additional Details : Weight : 0.25000kg
DescriptionDescription: The eBio4B10 monoclonal antibody reacts with mouse, rhesus moneky and human T-bet. T-bet is a Th1-specific T-box transcription factor critical to the development of the Th1 CD4+ T cell lineage. This is known based on the observations that T-Bet deficient mice have impaired Th1 cell development, and that ectopic expression of T-Bet results in development skewed to the Th1 lineage. T-Bet expression is induced by the Th1 cytokine IFN gamma, and T-Bet also regulates the expression of IFN gamma, likely, at least in part, through the modification of DNA accessibility and histone remodeling. In addition to IFN gamma, T-Bet is also known to regulate the expression of IL-12R beta and IL-2. Moreover, T-Bet plays a role in class-switch recombination in B-cells.Applications Reported: This eBio4B10 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This eBio4B10 antibody has been tested by intracellular staining and flow cytometric analysis of normal human peripheral blood cells using the Foxp3/Transcription Factor Staining Buffer Set (cat. 00-5523) and protocol. This antibody can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.For intracellular staining with the eBio4B10 monoclonal antibody, it is recommended to use the eBioscience Foxp3/Transcription Factor Staining Buffer Set (cat. 00-5523) and protocol. Please refer to Best Protocols: Protocol B: One step protocol for intracellular (nuclear) proteins).Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|T-PET, T-bet, TBET, TBLYM, T-box expressed in T cells, T-box protein 21, T-box transcription factor TBX21, T-cell-specific T-box transcription factor T-bet, transcription factor TBLYM|
|eBio4B10 (4B10), 4-B10)|
|IgG1, kappa, kappa|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
|30009, 57765, 694044|
For Research Use Only.