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Cocoon Biosciences T4 DNA Ligase

Produktkod. 31722998
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Koncentration:
2 MU/mL
400 kU/mL
Kvantitet:
100 kU
400 kU
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Produktkod. Koncentration Kvantitet
31722998 400 kU/mL 400 kU
31722997 2 MU/mL 100 kU
31722996 400 kU/mL 100 kU
31722999 2 MU/mL 400 kU
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Produktkod. 31722998 Leverantör Cocoon Biosciences Leverantörsnummer SP267126D1

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Beskrivning

Enables high-efficiency joining of DNA fragments, supporting cloning, assembly, and repair workflows. Its strong performance improves reaction reliability, boosts ligation success rates, and enhances overall workflow consistency.

  • Catalyzes the formation of phosphodiester bonds between cohesive or blunt ended strands of DNA or repairs single stranded nicks in duplex DNA.
  • Molecular cloning: used for ligation of DNA fragments into plasmid vectors, circularization of DNA molecules, and related applications.
  • DNA repair for joining double strand breaks: seals cohesive or blunt ends.
  • Joining adapters to DNA: ligates DNA fragments with specific adapters or vectors for large scale cloning and sequencing.
  • Source: Engineered Baculovirus carrying T4 DNA Ligase gene from T4 Bacteriophage inoculated in inoculate Trichoplusia ni.
  • Enzyme activity: One unit (U) is defined as the amount of enzyme required to ligate ≥90% of 3 μg of Lambda DNA-Hind III Digest in 20 min at 16°C.
  • Reliable and consistent quality: manufactured under high quality standards, ensuring batch to batch consistency.
  • High concentrated presentation for use in challenging assay conditions.
  • Delivered with 10X Ligation Buffer.
  • Environmentally considerate production prioritizing minimal environmental impact.
  • For research use or further manufacturing; not for diagnostic use or direct administration into humans or animals.

Specifikationer

Koncentration 400 kU/mL
Värd art Enterobacteria phage T4
Färg Transparent
Komponenter Ligation Buffer
Kvalitet RUO
Renhet >95% by SDS-PAGE
pH 7.4
För användning med (applikation) DNA Ligation, DNA repair
Förvaringsbuffert 10 mM Tris-HCl pH 7.4, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA and 50% Glycerol
Förpackning Vial with Lid
Innehåll och lagring -20°C
Uttryckssystem Trichoplusia ni (insect)
Gen-ID (Entrez) 1258680
Gene Alias Polydeoxyribonucleotide synthase [ATP]
Massa 57.5 kDa
Protein Tag His
Frakt skick 2°C to 8°C
Enzym DNA Ligase
Känslig för värmeinaktivering Yes
Kvantitet 400 kU
Produkttyp Enzyme
Forskningskategori Traditional Cloning, Next-generation Sequencing, DNA Repair
Molekylvikt (g/mol) 57455.41
Biologisk aktivitet DNA Ligase
Provtyp Purified Recombinant Protein
Form Liquid
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