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Applied Biosystems™ TaqMan™ Advanced miRNA Human Endogenous Controls Card

The Applied Biosystems TaqMan Advanced miRNA Human Endogenous Controls Card provides the convenience of pre-spotted TaqMan Advanced miRNA assays in a 384-well microfluidic card format.

Brand:  Applied Biosystems™ A34716

Additional Details : Weight : 0.05000kg

Product Code. 16669382

  • 3605.00 SEK / Each
Estimated Shipment: 19-08-2024
to see stock.

Description

Description

This product contains one card than can be used to evaluate 30 potential endogenous control miRNAs in triplicate for up to four samples. Two exogenous (non-human) controls are included for normalization of sample input.

Features of the TaqMan Advanced miRNA Human Endogenous Controls Card include:
Reproducibility—pre-spotted assays help reduce pipetting error and minimize experimental variability
Sample savings—requires only 2 μL purified plasma/serum or 1–10 ng total RNA per sample
Updated content—assay content updated for mirBase v.22
Easy to use—just add a mix of diluted miR-Amp and master mix and run the qPCR; no additional pipetting required
Gold-standard TaqMan qPCR chemistry—TaqMan Advanced miRNA assays draw on Thermo Fisher Scientific's industry-leading bioinformatics assay design pipeline and verification system to help ensure high specificity and minimal cross-reactivity, even for closely-related miRNA families

Product details:

Number of cards: 1
Number of unique miRNAs (not including exogenous controls): 30
Exogenous controls: cel-miR-39-3p, ath-miR159a
Compatible with the following Applied Biosystems qPCR instruments: Viia7, QuantStudio 7 Flex, and QuantStudio 12k Flex
Recommended master mix: TaqMan Fast Advanced Master Mix

Notes
• TaqMan Advanced miRNA cards require the use of the TaqMan Advanced cDNA Synthesis Kit, which uses a universal reverse transcription (RT) chemistry to prepare the cDNA template for use with TaqMan Advanced miRNA assays for detection and quantification of mature human miRNAs in biological samples.
• These plates are not compatible with the first-generation TaqMan MicroRNA cDNA Synthesis Kit, which uses a miRNA-specific reverse transcription chemistry.

Specifications

Specifications

One 384-well array

The TaqMan miRNA card is shipped at ambient temperature. Upon arrival, store at 2°C to 8°C and keep protected from light.
384-well Microfluidic Card
miRNA
miRNA Analysis
384
Endogenous Controls Card
Approved for shipment at Room Temperature or on Wet or Dry Ice
Primer-Probe Detection
Endogenous Control
Human
2 Color
TaqMan™
Fast or Standard
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For Research Use Only. Not for use in diagnostic procedures.