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Applied Biosystems™ TaqMan™ Genotyping Master Mix

Optimized for end-point fluorescence detection in SNP genotyping applications.

Brand:  Applied Biosystems™ 4371355

Additional Details : Weight : 0.02000kg

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Product Code. 10025894

  • 7740.00 SEK / Each


EU Stock 78
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For Research Use Only. All usage must comply with product instructions.


Description

Description

  • Distinct clusters and high call rates for unambiguous allelic discrimination
  • Validated with TaqMan™ SNP Genotyping Assays
  • Excellent pre- and post-PCR stability for high throughput setup and analysis
  • Reaction size is based on a 50μL reaction volume

May be used for a variety of genotyping applications, including:
  • Candidate gene studies
  • Drug-target validation
  • Disease association studies
  • Population genetics
  • Linkage mapping
  • Agricultural applications

Exceptional Performance from an Optimized Mix (2X)
Accurate genotype assignments result from preferential binding of the allele-specific probe to the matching target. TaqMan™ Genotyping Master Mix enables specific binding of the probe to achieve exceptional cluster resolution. Key features include:
  • AmpliTaq Gold™ DNA Polymerase, UP (Ultra Pure) - Automatic hot start enzyme designed to be active during thermal cycling and inactive at room temperature for easy reaction setup
  • Optimized mix components provide excellent specificity for discrimination between alleles
  • Passive internal reference based on proprietary ROX™ dye for increased precision on Applied Biosystems™ real-time PCR instruments
  • Single thermal cycling condition for consistent results with TaqMan™ Assays

Validated with TaqMan™ Genotyping Assays and Instruments:
  • TaqMan™ Genotyping Master Mix has been tested across all types of TaqMan™ SNP Genotyping Assays: TaqMan™ Drug Metabolism Genotyping Assays, TaqMan™ SNP Genotyping Assays, and Custom TaqMan™ SNP Genotyping Assays
  • In addition, the master mix is validated with the Applied Biosystems™ thermal cyclers and real-time PCR systems

High-Throughput Setup and Analysis:
  • The combination of several low-cost thermal cyclers for PCR and a single real-time PCR instrument for allelic discrimination make high throughput SNP genotyping manageable
  • TaqMan™ Genotyping Master Mix further improves high-throughput setup and analysis with excellent room temperature stability both before and after PCR
  • This provides the flexibility required to run long experiments, unattended overnight or over a weekend

Reliable Discrimination with Challenging Targets
  • Amplicon design constraints make SNP detection challenging, particularly when targets contain an abundance of GC- or AT-rich regions
  • The optimized components of TaqMan™ Genotyping Master Mix result in tight, well-separated clusters and more accurate allele-calls compared to other mixes, even for challenging targets

Setting the Industry Standard for Quality:
  • Extensive analytical and functional tests are performed for each manufactured lot of TaqMan™ Genotyping Master Mix, including one TaqMan™ SNP Genotyping Assay and one TaqMan™ Drug Metabolism Genotyping Assay
  • Results are compiled in an informative Certificate of Analysis, which is accessible through the Applied Biosystems™ Web site

Order Info

Guaranteed minimum shelf life is 60 days. Shipping Conditions: Wet Ice

Specifications

Specifications

Primer-Probe Detection
Genotyping & genomic profiling, pharma & biopharma, real time PCR (qPCR), target & lead identification & validation
Supplied at 2X concentration. The mix is optimized for SNP genotyping using TaqMan™ probes and contains AmpliTaq Gold™ DNA Polymerase UP (Ultra Pure), dNTPs without dUTP, Passive Reference 1 and optimized mix components. This pack contains one 10mL bottle, sufficient for 400 reactions at the 50μL total volume.
UP (Ultra Pure)
DNA (Genomic)
Store at 2° to 8°C.
Passive Reference Dye: ROX (Pre-mixed)
For use with QuantStudio™ 12K Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6, QuantStudio™ 7
AmpliTaq Gold™ DNA Polymerase
1 x 10mL
Standard
Copy number variants, Drug metabolizing enzymes, SNPs (Known), SNPs (Unknown or Numerous)
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Special Offers

Special Offers

  • Save up to 61% for a limited time only
    : 13834

    2019-11-29

    Discount will apply to qualifying orders received by Fisher Scientific between 01/09/19 and 31/12/19. Promotion code must be presented at the time of order. Discount applies to the list price in effect at the time the order is received. Promotion cannot be combined with other discounts or promotions. Offer void where prohibited, licensed, or restricted by federal, state, provincial, or local laws or regulation or agency/institutional policy. Other restrictions may apply.

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For Research Use Only. Not for use in diagnostic procedures.