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Invitrogen™ Human Tau (Phospho) [pT181] ELISA Kit

Product Code. 10591255
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Kvantitet:
96 tester
Unit Size:
96 tester
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10591255 96 tester 96 tester
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For Research Use Only. All usage must comply with product instructions.
Product Code. 10591255 Supplier Invitrogen™ Supplier No. KHO0631
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For Research Use Only. All usage must comply with product instructions.

Description

ELISA

The Human Tau (Phospho) [pT181] ELISA Kit is a solid-phase sandwich Enzyme-Linked Immunosorbent Assay (ELISA) designed to detect and quantify the level of Tau (Phospho) [pT181] in human cerebrospinal fluid, tissue homogenates, buffered solutions and cell culture supernatants. The assay recognizes both natural and recombinant Tau (Phospho) [pT181]. Principle of the method A monoclonal capture antibody specific for Tau (Phospho) [pT181] has been coated onto the wells of the 96-well plate. During the first incubation, standards of known content and unknown samples are pipetted into the wells and the antigen binds to the immobilized (capture) antibody. After washing, a rabbit antibody specific for the target protein is added to the wells and serves as a detection antibody by binding to the immobilized protein captured during the first incubation. After washing, a horseradish peroxidase labeled anti-rabbit IgG is added. This binds to the detection antibody to complete the four member sandwich. After a third incubation and washing to remove all the unbound enzyme, a substrate solution (TMB) is added, which is acted upon by the bound enzyme to produce color. The intensity of this colored product is directly proportional to the concentration of target protein present in the original specimen and the optical density can be read on a standard microplate reader. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for cri...

Tau is a neuronal microtubule-associated protein found predominantly on axons. The function of Tau is to promote tubulin polymerization and stabilize microtubules. The C-terminus binds axonal microtubules while the N- terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton while the longer isoforms may preferentially play a role in its stabilization. In its hyper-phosphorylated form, Tau is the major component of paired helical filaments (PHF), the building block of neurofibrillary lesions in Alzheimer's diseases (AD) brain. Hyper-phosphorylation impairs the microtubule binding function of Tau, resulting in the destabilization of microtubules in AD brains, ultimately leading to the degeneration of the affected neurons. Numerous serine/threonine kinases phosphorylate Tau, including GSK-3beta, protein kinase A (PKA), cyclin-dependent kinase 5 (cdk5) and casein kinase II. Hyper-phosphorylated Tau is found in neurofibrillary lesions in a range and other central nervous system disorders such as Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy.
TRUSTED_SUSTAINABILITY

Specifications

Tillträdesnummer P10636
Analysintervall 15,6 till 1000 pg/ml
Analyskänslighet <10 pg/mL
Koncentration 15.6 to 1000 pg/mL
Innehåll och lagring Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.
Konjugera HRP
Immunoassay Kit Format Sandwich ELISA Kit
Produkttyp ELISA
Provtyp Cerebrospinalvätska, Supernatant, Vävnadshomogenater
Målarter Människa
För användning med (utrustning) Kolorimetrisk mikroplattläsare
Gene Alias AI413597; AW045860; DDPAC; FLJ31424; FTDP17; FTDP-17; G protein beta1/gamma2 subunit-interacting factor 1; map tau; Mapt; MAPTL; MGC138549; microtubule associated protein tau; microtubule-associated protein tau; microtubule-associated protein tau, isoform 4; microtubules; MSTD; Mtapt; MTBT1; MTBT2; Neurofibrillary tangle protein; neurofibrillary tangles; Neuronal Marker; paired helical filament-tau; PHFtau; PHF-tau; PPND; PPP1R103; protein phosphatase 1, regulatory subunit 103; pTau; RNPTAU; Tau; Tau microtubule-associated protein; tau protein; Tau-4; Tau5; Unknown (protein for MGC:134287)AI413597; AW045860; DDPAC; FLJ31424; FTDP17; FTDP-17; G protein beta1/gamma2 subunit-interacting factor 1; map tau; Mapt; MAPTL; MGC138549; microtubule associated protein tau; microtubule-associated protein tau; microtubule-associated protein tau, isoform 4; microtubules; MSTD; Mtapt; MTBT1; MTBT2; Neurofibrillary tangle protein; neurofibrillary tangles; Neuronal Marker; paired helical filament-tau; PHFtau; PHF-tau; PPND; PPP1R103; protein phosphatase 1, regulatory subunit 103; pTau; RNPTAU; Tau; Tau microtubule-associated protein; tau protein; Tau-4; Tau5; Unknown (protein for MGC:134287); DDPAC; FTDP-17L; MSTD; MTBT1; MTBT2; PPND; PPP1R103; TAU; MAPT
Gen-ID (Entrez) 4137
Gensymbol MAPT
Inkubationstid 4 hr.
Interassay CV 5.7%
Intraassay CV 4.3%
Kit Innehåll Förbelagd 96 brunnars platta, Standard, Standard utspädningsbuffert, Detektionsantikropp, anti-rabbit-HRP, HRP-spädningsmedel, Tvättbuffert, Kromogen, Stoppa lösning, Självhäftande plåtskydd
Etikett eller färgämne HRP
Markör Gene Tau
Proteinaliaser Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau
Kvantitet 96 tester
Regulatorisk status RUO
Provvolym Cerebrospinalvätska 10μ L, Supernatant 10μ L, vävnadshomogenat 10μ L
Frakt skick Wet Ice
Förvaringskrav 2 °C till 8 °C
Mål Tau
Testtid 1 tim 20 min
Total analystid 15 hr
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Safety and Handling

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Product Identifier
  • Human Tau (Phospho) [pT181] ELISA Kit
 Signal Word
  • Varning
 Hazard Category
  • Akut toxicitet Kategorija 4
  • FARA FÖR SKADLIGA LÅNGTIDSEFFEKTER (FÖR VATTENMILJÖN) Kategori: kronisk 3
 Hazard Statement
  • H302-Skadligt vid förtäring.
  • H412-Skadliga långtidseffekter för vattenlevande organismer.
 Precautionary Statement
  • P264-Tvätta grundligt efter användning.
  • P270-Ät inte, drick inte och rök inte när du använder produkten.
  • P273-Undvik utsläpp till miljön.
  • P301+P310-VID FÖRTÄRING: Kontakta genast GIFTINFORMATIONSCENTRALEN/läkare
  • P330-Skölj munnen.
  • P501b-Innehållet/behållaren lämnas till I enlighet med lokala / regionala / nationella / internationella bestämmelser (ska anges).
 Supplemental information
  • MIXTURE LIST-Contain: Sodium azide
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