TCR V beta 9 Monoclonal Antibody (AMKB1-2), APC, eBioscience™, Invitrogen™

Description

Description: This AMKB1-2 monoclonal antibody recognizes the human T cell receptor (TCR) Vbeta9 allele. Composed of an alpha and beta chain, TCR specificity is typically determined by Valpha, Jalpha, Vbeta, Dbeta, and Jbeta gene rearrangement. Vbeta expression in humans has been examined in studies on the effects of superantigens, inflammation, autoimmune disease, and HIV infection. More recently, assessment of TCR Vbeta expression has been used to phenotype T cell clonality in CD3^+/TCRalpha beta^+ large granular lymphocyte leukemias. A member of the Ig superfamily, this receptor is expressed on a subset of peripheral blood T cells. Applications Reported: This AMKB1-2 antibody has been reported for use in flow cytometric analysis. Applications Tested: This AMKB1-2 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser. Filtration: 0.2 μm post-manufacturing filtered.

The ability of T cell receptors (TCR) to discriminate foreign from self-peptides presented by major histocompatibility complex (MHC) class II molecules is essential for an effective adaptive immune response. TCR recognition of self-peptides has been linked to autoimmune disease. Mutant self-peptides have been associated with tumors. Engagement of TCRs by a family of bacterial toxins know as superantigens has been responsible for toxic shock syndrome. Autoantibodies to V beta segments of T cell receptors have been isolated from patients with rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE). The autoantibodies block TH1-mediated inflammatory autodestructive reactions and are believed to be a method by which the immune system compensates for disease. Most human T cells express the TCR alpha-beta and either CD4 or CD8 molecule (single positive, SP). A small number of T cells lack both CD4 and CD8 (double negative, DN). Increased percentages of alpha-beta DN T cells have been identified in some autoimmune and immunodeficiency disorders. Gamma-delta T cells are primarily found within the epithelium. They show less TCR diversity and recognize antigens differently than alpha-beta T cells. Subsets of gamma-delta T cells have shown antitumor and immunoregulatory activity.