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TER-119, PE-Cyanine7, clone: TER-119, eBioscience™

Rat Monoclonal Antibody

1044.00 SEK - 1676.00 SEK

Specifications

Antigen TER-119
Clone TER-119
Target Species Murine
Conjugate PE-Cyanine7
Isotype IgG2b, kappa, kappa
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Products 2
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
15330990 Affymetrix eBioscience
25-5921-81
50μg 1044.00 SEK
50µg
EU Stock 1
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15340990 Affymetrix eBioscience
25-5921-82
100μg 1676.00 SEK
100µg
EU Stock 5
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Description

Description

Description: The TER-119 monoclonal antibody reacts with mouse erythroid cells from early proerythroblast to mature erythrocyte stages. The TER-119 antigen is present in yolk sac, fetal and newborn liver, but is not expressed by cells carrying BFU-E and CFU-E activities. Several erythroleukemia cell lines tested so far are negative for expression of TER-119 antigen even after dimethylsulfoxide stimulation. Biochemical and molecular analysis of the TER-119 antigen indicate that this molecule is associated with the surface glycophorin A, but is not a typical glycophorin.Applications Reported: This TER-119 antibody has been reported for use in flow cytometric analysis.Applications Tested: This TER-119 antibody has been tested by flow cytometric analysis of mouse splenocytes and bone marrow cells. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

TER-119
Murine
IgG2b, kappa, kappa
Monoclonal
Liquid
Antibody
4° C, store in dark, DO NOT FREEZE!, handling and experimental procedures.
TER-119
PE-Cyanine7
Flow Cytometry
Primary
Monoclonal
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
RUO

For Research Use Only.