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Thermo Scientific™ Reagents for GeneJET™ Plasmid Miniprep Kit

Components of the GeneJET Plasmid Miniprep Kit (K0502/K0503) that may be purchased separately.

358.00 SEK - 584.00 SEK

Specifications

Content And Storage Store at room temperature,-20°C
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Products 6
Product Code Brand Description Quantity Price Quantity & Availability  
Product Code Brand Description Quantity Price Quantity & Availability  
10397979
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Thermo Scientific™
R1223
Lysis Solution 70 mL
500.00 SEK
70mL
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10669340
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Thermo Scientific™
R1213
Reagent 70 mL
524.00 SEK
70mL
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10202510
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Thermo Scientific™
R1253
Reagent 0.7 mL
358.00 SEK
0.70mL
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10307989
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Thermo Scientific™
R1233
Neutralization Solution 100 mL
520.00 SEK
100mL
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10881361
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Thermo Scientific™
R1243
Reagent 100 mL
582.00 SEK
100mL
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10796031
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Thermo Scientific™
R1331
Reagent 150 mL
584.00 SEK
150mL
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Estimated Shipment: 22-04-2024
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Description

Description

Thermo Scientific™ GeneJET™ Neutralization Solution and Lysis Solution are components of the GeneJET Plasmid Miniprep Kit (K0502/K0503) and may be purchased separately.

The kit utilizes an exclusive silica-based membrane technology in the form of a convenient spin column. The kit recovers up to 20μg of high copy plasmid DNA per isolation procedure.

The Thermo Scientific GeneJET Plasmid Miniprep Kit utilizes an exclusive silica-based membrane technology in the form of a convenient spin column. The kit recovers up to 20 μg of high copy plasmid DNA per isolation procedure.

Highlights
  • Efficient - high yields of up to 20 μg of high quality plasmid DNA
  • Fast - procedure takes less than 14 minutes
  • Convenient - no phenol/chloroform extraction or alcohol precipitation required
  • Pure - purified DNA is immediately ready to use
Applications
Fast isolation of high purity plasmid DNA suitable for all conventional molecular biology procedures, including:
  • FastDigest or conventional restriction digestion
  • Automated fluorescent and radioactive sequencing
  • PCR
  • In vitro transcription
  • Transformation
Principle
A bacterial culture is harvested and lysed. The lysate is then cleared by centrifugation and applied on the silica column to selectively bind DNA molecules at a high salt concentration. The adsorbed DNA is washed to remove contaminants, and the pure plasmid DNA is eluted in a small volume of elution buffer or water. The purified DNA is ready for immediate use in all molecular biology procedures, such as fast and conventional digestion with restriction enzymes, PCR, in vitro transcription, transformation, and automated sequencing.

Components
  • Resuspension Solution
  • Lysis Solution
  • Neutralization Solution
  • Wash Solution (concentrated)
  • RNase A Solution
  • Elution Buffer
  • Spin Columns
  • Collection Tubes
  • Detailed Protocol

Hazardous: No
Quality Control:

  • The kit is tested for the isolation of pUC19 DNA from E. coli according to the protocol.
  • The quality of the isolated DNA is evaluated by spectrophotometric assay, agarose gel electrophoresis, digestion with FastDigest restriction enzymes, and automated sequencing.
Storage Condition: Ambient,-20°C

Fast isolation of high purity plasmid DNA suitable for all conventional molecular biology procedures, including:—FastDigest or conventional restriction digestion,—automated fluorescent and radioactive sequencing

Order Info

Orders placed Monday through Wednesday ship next day; Thursday and Friday orders ship the following Monday.

Specifications

Specifications

Store at room temperature,-20°C
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