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Invitrogen™ Human TNF alpha Matched Antibody Pair

Produktkod. 10353643
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Kvantitet:
10 x 96 Tests
Förpackningsstorlek:
Varje
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10353643 10 x 96 Tests Varje
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For Research Use Only. All usage must comply with product instructions.
Produktkod. 10353643 Leverantör Invitrogen™ Leverantörsnummer CHC1753
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For Research Use Only. All usage must comply with product instructions.

Beskrivning

ELISA

The Human Tumor Necrosis Factor alpha Antibody Pair Kit comes with pre-matched antibody pairs, standards, and streptavidin-HRP to develop your own enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human TNFα. Buffer reagents needed to complete the ELISA reaction are sold separate as a Buffer Kit for Antibody Pairs (Catalog No. CNB0011). The Assay Buffer included in this Buffer Kit can be used as a blocking reagent for ELISA plates as well as a diluent for ELISA standards and samples, detection antibody, and HRP conjugate. Principle of the method ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.
TRUSTED_SUSTAINABILITY

Specifikationer

Tillträdesnummer P01375
Analysintervall 15.6-1000 pg/mL
Analyskänslighet <15.6 pg/mL
Konjugera HRP
Genaccessionsnr. P01375
Gene Alias DIF, TNF-alpha, TNFA, TNFSF2, APC1 protein; Cachectin; C-domain 1; C-domain 2; cTNF; DADB-70P7.1; DIF; ICD1; ICD2; Intracellular domain 1; Intracellular domain 2; N-terminal fragment; NTF; RATTNF; Tnf; TNF alpha; TNF superfamily; TNF α; TNF, macrophage-derived; TNF, monocyte-derived; TNFA; TNF-a; TNFalpha; TNF-alpha; Tnfsf1a; TNFSF2; TNFα; TNLG1F; Tumor necrosis factor; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; tumor necrosis factor alpha (cachetin); tumor necrosis factor alpha precursor; tumor necrosis factor ligand 1F; tumor necrosis factor ligand superfamily member 2; Tumor necrosis factor, membrane form; Tumor necrosis factor, soluble form; tumor necrosis factor-alpha; tumor necrosis factor-alpha precursor; tumor-necrosis factor; tumour necrosis factorAPC1 protein; Cachectin; C-domain 1; C-domain 2; cTNF; DADB-70P7.1; DIF; ICD1; ICD2; Intracellular domain 1; Intracellular domain 2; N-terminal fragment; NTF; RATTNF; Tnf; TNF alpha; TNF superfamily; TNF α; TNF, macrophage-derived; TNF, monocyte-derived; TNFA; TNF-a; TNFalpha; TNF-alpha; Tnfsf1a; TNFSF2; TNFα; TNLG1F; Tumor necrosis factor; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; tumor necrosis factor alpha (cachetin); tumor necrosis factor alpha precursor; tumor necrosis factor ligand 1F; tumor necrosis factor ligand superfamily member 2; Tumor necrosis factor, membrane form; Tumor necrosis factor, soluble form; tumor necrosis factor-alpha; tumor necrosis factor-alpha precursor; tumor-necrosis factor; tumour necrosis factor; DIF-alpha; TNFA; TNFSF2; TNLG1F; TNF
Gensymboler TNF
Immunoassay Kit Format Matched Antibody Pair
Produkttyp ELISA, ELISA Kit
Kvantitet 10 x 96 Tests
Provtyp Plasma, Serum, Supernatant
Regulatorisk status RUO
Gen-ID (Entrez) 7124
Målarter Human
För användning med (utrustning) Absorbance Microplate Reader, Colorimetric Microplate Reader
Inkluderar Capture (coating) Antibody, Detection Antibody, Standard, Streptavidin-HRP
Innehåll och lagring Antibody pair Kit contains matched, pre-titred and fully optimized Capture (coating) and Detection Antibodies, 3 vials of Recombinant Standard, Streptavidin-HRP concentrate and technical data sheet.
Store at 2-8°C.
Gensymbol Tnf
Kit Innehåll Capture (coating) Antibody, Detection Antibody, Standard, Streptavidin-HRP
Etikett eller färgämne Biotin
Frakt skick Wet Ice
Förvaringskrav 2-8°C
Mål TNF alpha
Testtid 1 hr 30 min
Total analystid 24 hr
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