TNF alpha, clone: MAb1, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 14-7348-85
Additional Details : Weight : 0.01000kg
DescriptionDescription: The MAb1 antibody reacts with human tumor necrosis factor-alpha (TNF-alpha), a 17kDa cytokine produced by monocytes, macrophages, neutrophils, NK cells and CD4^+ T cells. TNF-alpha has cytolytic activity against a range of tumor cells and is important in immune regulation. TNF-alpha forms dimers and trimers and also exists as a 26kDa membrane bound form.Applications Reported: MAb1 has been reported for use in capture of human TNF alpha by ELISA. It can also be used for immunoblotting (WB) and for neutralizing TNF alpha binding and biological activity. (Please use Functional Grade purified MAb1, cat. 16-7348, in functional assays.).Applications Tested: The MAb1 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of human TNF alpha in combination with the biotin MAb11 (13-7349) antibody for detection and recombinant human TNF alpha (14-8329) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 μg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/mL - 8 pg/mL should be included in each ELISA plate.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.09% sodium azide; pH 7.2|
|DADB-70P7.1, DIF, TNF-alpha, TNFA, TNFSF2|
|ELISA, Dot blot|
|IgG1, kappa, kappa|
For Research Use Only.