TNF alpha, clone: MP6-XT22, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 14-7321-85
Additional Details : Weight : 0.01000kg
DescriptionDescription: The MP6-XT22 antibody reacts with mouse tumor necrosis factor-alpha (TNF alpha), a 17kDa cytokine produced by monocytes, macrophages, neutrophils, NK cells and CD4(+)T cells. TNF alpha has cytolytic activity against a range of tumor cells and is important in immune regulation. TNF alpha forms dimers and trimers and also exists as a 26kDa membrane-bound form.Applications Reported: This MP6-XT22 antibody has been reported for use in intracellular staining followed by flow cytometric analysis, and immunohistology staining of frozen tissue sections. (Fluorochrome conjugated MP6-XT22 is recommended for use in intracellular flow cytometry.).Applications Tested: The MP6-XT22 antibody has been tested to block staining with fluorochrome conjugated MP6-XT22. This can be used at less than or equal to 2 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.09% sodium azide; pH 7.2|
|DADB-70P7.1, DIF, TNF-alpha, TNFA, TNFSF2|
|Flow CytometryþImmunohistochemistry (Frozen)|
|Store at 2-8°C.|
For Research Use Only.