TRA-1-60 (Podocalyxin) Mouse anti-Human, Clone: TRA-1-60, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 14-8863-80
Additional Details : Weight : 0.01000kg
DescriptionCell number should be determined empirically but can range from 10^5 to 10^8 cells/test. This antibody has also been tested by immunoblotting of 2102Ep cell extracts, yielding a band of approximately 200-250 kDa (reducing and non-reducing conditions). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered. TRA-1-60 is a cell surface antigen, expressed along with SSEA-3, SSEA-4 and TRA-1-81 in human embryonic stem cells, embryonal carcinoma cells and induced pluripotent stem cells (iPS). These surface markers are lost during the differentiation process. In contrast, SSEA-1 is absent in undifferentiated human stem cells but is present on the cell surface after retinoic acid mediated differentiation.
|PBS with 0.09% sodium azide; pH 7.2|
|Keratan sulfate antigen TRA1-60; TRA160|
|Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Western Blot|
For Research Use Only.