TSLP, Functional Grade, clone: eBio28F12 (28F12), eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 16-5491-82
Additional Details : Weight : 0.01000kg
DescriptionDescription: The monoclonal antibody eBio28F12 reacts with and inhibits the bioactivity of mouse TSLP. Thymic Stromal-Derived Lymphopoetin (TSLP) was first identified in the tissue culture supernatant of a thymic stromal cell line as a growth factor capable of inducing proliferation and differentiation of pre-B cells. It was later found to be closely related to another stromal cytokine, IL-7, with which it shares overlapping functions. Along with its proliferative effects on B cells, TSLP induces dendritic cells to support the differentiation of naive T cells towards the Th2 lineage and may also be involved in the development of CD4^+CD25^+ regulatory T cells. The receptor for TSLP is heterodimeric and shares one subunit, IL-7Ralpha, with IL-7 and other members of the family, while the TSLPR subunit is unique to TSLP. TSLP expression can be induced by a variety of inflammatory cytokines and TLR ligands. Expression is regulated by NF-kappaB and has been detected in epithelial cells, stromal cells, and basophils. Elevated levels of TSLP are associated with asthma and atopic dermatitis, which are both Th2-mediated inflammatory conditions. It is believed that chronic overexpression of TSLP may result in increased sensitivity to allergens, resulting in susceptibility to these conditions. Applications Reported: The monoclonal antibody eBio28F12 reacts with and inhibits the bioactivity of mouse TSLP.Applications Tested: The ND50 of eBio28F12, as determined by the neutralization of recombinant mouse TSLP-induced proliferation of the Nag8/7 cell line, is 1-3 ng/mL in the presence of 0.5 ng/mL mTSLP. The neutralization dose of this antibody will vary depending on cell type, assay method, and cytokine concentration.Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay.Storage and handling: Use in a sterile environment.Filtration: 0.2μm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.
|PBS with no preservative; pH 7.2|
|thymic stroma-derived lymphopoietin, thymic stromal lymphopoietin|
|Functional Assay, Neutralization|
|IgG2a, kappa, kappa|
For Research Use Only.