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TCR V alpha 2 Rat anti-Mouse, Biotin, Clone: B20.1, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 13-5812-82

Additional Details : Weight : 0.25000kg

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Product Code. 15239869

  • 1016.00 SEK / 100µg

Estimated Shipment: 27-07-2021

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Description

Description

Description: The B20.1 monoclonal antibody reacts with mouse T-cell receptor (TCR) V alpha 2 chains. This antibody recognizes the majority of the TCR V alpha 2 subfamily in mice carrying the a, b and c haplotypes. The antibody also reacts with the products of T cell receptor, V delta 8 due to the high degree of homology. Applications Reported: This B20.1 antibody has been reported for use in flow cytometric analysis and immunoprecipitation. Applications Tested: This B20.1 antibody has been tested by flow cytometric analysis of mouse lymph node cells. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended to preblock Fc receptors for 10 minutes using 0.5 μg of purified anti-mouse CD16/32 (cat. 14-0161). It is also recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Filtration: 0.2 μm post-manufacturing filtered.

The ability of T cell receptors (TCR) to discriminate foreign from self-peptides presented by major histocompatibility complex (MHC) class II molecules is essential for an effective adaptive immune response. TCR recognition of self-peptides has been linked to autoimmune disease. Mutant self-peptides have been associated with tumors. Engagement of TCRs by a family of bacterial toxins know as superantigens has been responsible for toxic shock syndrome. Autoantibodies to V beta segments of T cell receptors have been isolated from patients with rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE). The autoantibodies block TH1-mediated inflammatory autodestructive reactions and are believed to be a method by which the immune system compensates for disease. Most human T cells express the TCR alpha-beta and either CD4 or CD8 molecule (single positive, SP). A small number of T cells lack both CD4 and CD8 (double negative, DN). Increased percentages of alpha-beta DN T cells have been identified in some autoimmune and immunodeficiency disorders. Gamma-delta T cells are primarily found within the epithelium. They show less TCR diversity and recognize antigens differently than alpha-beta T cells. Subsets of gamma-delta T cells have shown antitumor and immunoregulatory activity.
Specifications

Specifications

TCR V alpha 2
Monoclonal
0.5 mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
TCRVA; TCRVA2; V alpha 2; V alpha-2 TCR; Valpha 2; Valpha2; Va 2; Va2
IgG2a, lambda
100 μg
4° C, store in dark, DO NOT FREEZE!
Primary
Mouse
Flow Cytometry, Immunoprecipitation
B20.1
Biotin
Liquid
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
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