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Invitrogen™ Human VCAM-1 ELISA Kit

Produktkod. 10029322
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Kvantitet:
96 tester
Förpackningsstorlek:
96 tester
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10029322 96 tester 96 tester
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For Research Use Only. All usage must comply with product instructions.
Produktkod. 10029322 Leverantör Invitrogen™ Leverantörsnummer KHT0601
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Beräknad leverans: 05-08-2026
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For Research Use Only. All usage must comply with product instructions.

Beskrivning

ELISA

The Human Vascular Cell Adhesion Molecule 1 (Hu VCAM-1) ELISA quantitates Hu VCAM-1 in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu VCAM-1. Principle of the method The Human VCAM-1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

VCAM-1 is a cell surface sialoglycoprotein expressed by cytokine activated endothelium. The protein has a number of functions including the regulation of leukocyte migration, leukocyte-endothelial cell adhesion and signal transduction and may play a role in a number of inflammatory diseases.
TRUSTED_SUSTAINABILITY

Specifikationer

Tillträdesnummer P19320
Analysintervall 0,59 till 75 ng/ml
Analyskänslighet <0.5 ng/mL
Innehåll och lagring Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.
Konjugera HRP
Immunoassay Kit Format Sandwich ELISA Kit
Produkttyp ELISA
Provtyp Plasma, Serum, Supernatant
Målarter Människa
För användning med (applikation) Kolorimetrisk detektion
För användning med (utrustning) Kolorimetrisk mikroplattläsare
Gene Alias adhesion molecule VCAM-1; CD106; CD106 antigen; DKFZp779G2333; INCAM100; INCAM-100; MGC99561; sCD106; soluble CD106; soluble VCAM 1; sVCAM 1; vascular cell adhesion molecule; vascular cell adhesion molecule 1; vascular cell adhesion molecule-1; vascular cell adhesion protein 1; V-CAM 1; Vcam1; Vcam-1; VCAM1Badhesion molecule VCAM-1; CD106; CD106 antigen; DKFZp779G2333; INCAM100; INCAM-100; MGC99561; sCD106; soluble CD106; soluble VCAM 1; sVCAM 1; vascular cell adhesion molecule; vascular cell adhesion molecule 1; vascular cell adhesion molecule-1; vascular cell adhesion protein 1; V-CAM 1; Vcam1; Vcam-1; VCAM1B; CD106; INCAM-100
Gen-ID (Entrez) 7412
Gensymbol CD106, INCAM-100
Inkubationstid 3 hr.
Interassay CV 6.4%
Intraassay CV 6.7%
Kit Innehåll Förbelagd 96 brunnars platta, Standard, Standard utspädningsbuffert, Biotinylerad detektionsantikropp, Streptavidin-HRP, HRP-spädningsmedel, Tvättbuffert, Kromogen, Stoppa lösning, Självhäftande plåtskydd
Etikett eller färgämne Biotin
Markör Gene VCAM-1
Proteinaliaser V-CAM 1, VCAM-1, INCAM-100
Kvantitet 96 tester
Regulatorisk status RUO
Provvolym Plasma 10μ L, Serum 10μ L, Supernatant 10μ L
Frakt skick Wet Ice
Förvaringskrav 2 °C till 8 °C
Mål VCAM-1 (CD106)
Testtid 1 tim 20 min
Total analystid 3 hr
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