VEGF Receptor 1 (Flt1), clone: 7A6, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 14-5936-80
Additional Details : Weight : 0.01000kg
DescriptionDescription: This 7A6 monoclonal antibody recognizes human VEGFR1 (vascular endothelial growth factor receptor 1, fms-like tyrosine kinase 1, FLT1). It is directed against the extracellular domain and can recognize both membrane-bound and soluble forms of VEGFR1, with approximate molecular weights of 180kDa and 90 kDa, respectively. Mice lacking expression of VEGFR1 have disorganized vasculature and die in utero, however angiogenesis does not require the tyrosine kinase activity of VEGFR1 as expression of a membrane-bound form lacking the tyrosine kinase domain allows normal development. Although fertile and viable, mice lacking the tyrosine kinase domain of VEGFR1 do have a defect in the migration of macrophages. The soluble form of VEGFR1 can be detected in the serum of normal patients, and elevated levels of soluble VEGFR1 in pregnant women is associated with preeclampsia. Several tumor cell lines have been reported to express membrane-bound VEGFR1, including malignancies of hematopoietic origin.Applications Reported: This 7A6 antibody has been reported for use in immunoblotting (WB).Applications Tested: This 7A6 antibody has been tested by immunoblot analysis of HUVEC. It can be used at 1-5 μg/mL. This antibody only recognizes non-reduced epitopes. It is highly recommended to include protease inhibitors in the lysing buffer and to not boil lysates prior to separation by non-reducing SDS-PAGE.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
|VEGF Receptor 1 (Flt1)|
|PBS with 0.09% sodium azide; pH 7.2|
|Store at 2-8°C.|
For Research Use Only.