VEGF Receptor 1 (Flt1) Monoclonal Antibody (7A6), eBioscience™, Invitrogen™

Description

Description: This 7A6 monoclonal antibody recognizes human VEGFR1 (vascular endothelial growth factor receptor 1, fms-like tyrosine kinase 1, FLT1). It is directed against the extracellular domain and can recognize both membrane-bound and soluble forms of VEGFR1, with approximate molecular weights of 180 kDa and 90 kDa, respectively. Mice lacking expression of VEGFR1 have disorganized vasculature and die in utero, however angiogenesis does not require the tyrosine kinase activity of VEGFR1 as expression of a membrane-bound form lacking the tyrosine kinase domain allows normal development. Although fertile and viable, mice lacking the tyrosine kinase domain of VEGFR1 do have a defect in the migration of macrophages. The soluble form of VEGFR1 can be detected in the serum of normal patients, and elevated levels of soluble VEGFR1 in pregt women is associated with preeclampsia. Several tumor cell lines have been reported to express membrane-bound VEGFR1, including maligcies of hematopoietic origin. Applications Reported: This 7A6 antibody has been reported for use in immunoblotting (WB). Applications Tested: This 7A6 antibody has been tested by immunoblot analysis of HUVEC. It can be used at 1-5 μg/mL. This antibody only recognizes non-reduced epitopes. It is highly recommended to include protease inhibitors in the lysing buffer and to not boil lysates prior to separation by non-reducing SDS-PAGE. Purity: Greater than 90%, as determined by SDS-PAGE.

FLT1 (VEGFR1) is a receptor tyrosine kinase which plays a critical role in angiogenesis. Active angiogenesis has been found to play important role in many pathological and physiological processes, such as tumor progression, diabetic retinopathy, rheumatoid arthritis and wound healing. May play an essential role as a negative regulator of embryonic angiogenesis by inhibiting excessive proliferation of endothelial cells. Can promote endothelial cell proliferation, survival and angiogenesis in adulthood. Its function in promoting cell proliferation seems to be cell-type specific. Promotes PGF-mediated proliferation of endothelial cells, proliferation of some types of cancer cells, but does not promote proliferation of normal fibroblasts (in vitro). Has very high affinity for VEGFA and relatively low protein kinase activity; may function as a negative regulator of VEGFA signaling by limiting the amount of free VEGFA and preventing its binding to KDR.