Vimentin, clone: V9, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 14-9897-80
Additional Details : Weight : 0.01000kg
DescriptionDescription: The V9 monoclonal antibody recognizes human Vimentin, a 57kDa protein that functions as a structural component of intermediate filaments. Vimentin is expressed in cells derived from the mesenchyme but also in specific populations such as radial glia and immature glial cells, pancreatic precursor cells. It is proposed to be a marker of cardiac differentiation. In neural cells, vimentin expression is gradually replaced by neurofilaments. Reports have also shown surface expression of vimentin on activated macrophages, platelets, as well as apoptotic T cells and neutrophils.This antibody also recognizes canine (dog), rat and chicken vimentin but does not recognize mouse vimentin.Applications Reported: This V9 antibody has been reported for use in western blotting, immunocytochemistry, and immunohistochemical staining of frozen (IHC-F) and formalin-fixed paraffin embedded tissue sections (IHC-P).Applications Tested: This V9 antibody has been tested by immunocytochemistry on paraformaldehyde fixed and permeabilized SK-N-SH or C6 cell lines and by western blot on cell lysates prepared from HeLa cells. This antibody can be used at less than or equal to 1μg/ml. It is recommended that this antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.09% sodium azide; pH 7.2|
|P09654, P31000, P08670|
|ImmunocytochemistryþImmunohistochemistryþImmunohistochemistry (Frozen)þWestern Blot|
|Store at 2-8°C.|
For Research Use Only.