Thermo Scientific™ Zeba™ Spin Desalting Columns and Plates, 40K MWCO, 75 μL–10 mL

Thermo Scientific Zeba Spin Desalting columns and plates (40K MWCO) are convenient, ready-to-use devices compatible with major automated liquid-chromatography systems or for manual syringe processing. The polypropylene columns have a 40K MWCO and contain a proprietary high-performance size-exclusion chromatography resin (Zeba High-Performance Resin) that offers excellent protein desalting and recovery in a centrifuge format. When using Zeba Micro Spin Desalting Columns, 40K MWCO, 75 μL, even very dilute (25 μg/mL) protein samples can be successfully processed to obtain greater than 95% retention (removal) of salts and other small molecules (<2000 MW), and good recovery of proteins and other macromolecules (>40,000 MW).

Zeba Spin Desalting Plates are polypropylene devices containing a proprietary high-performance size-exclusion resin for effective removal of salts and other small molecules (<2000 daltons) while providing excellent recovery for proteins >40,000 Da. They provide trouble-free desalting and buffer exchange for sample volumes ranging from 20 to 100 μL. This 96-well spin plate format enables more high-throughput processing.

The Zeba 96-well spin desalting plates require no resin dispensing or hydration and provide the same high protein recovery as spin columns with at least 95% removal of salts and other small molecules. One plate can process 96 small sample volumes (20 to 100 μL) in five minutes.

Benefits of Zeba Spin Desalting columns and plates include:
• High recovery—low-binding resin maximizes protein recovery
• Fast—no fraction screening or waiting for protein to emerge by gravity flow
• Economical—great performance at lower cost than other commercially available cartridges
• Easy-to-use—no cumbersome column preparation or equilibration
• Flexible—available in spin columns and filter spin plates for a range of needs

Available Formats
• Spin columns—75 μL, 0.5 mL, 2 mL, 5 mL, 10 mL columns
• 96-well spin plates—pre-dispensed 96-well filter plates, compatible with centrifugation for manual or automated purification