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Invitrogen™ Zenon™ Rabbit IgG Labeling Kits Product Code.: 10013402

Invitrogen™ Zenon™ Rabbit IgG Labeling Kits

Product Code. 10013402
50 Reactions kit, 578/603 nm, Alexa Fluor 568, Each
Click to view available options
Quantity:
25 Reactions
25 Reactions kit
3 x 10 ÎĽL
50 Reactions
50 Reactions kit
Excitation/Emission:
346, 495, 590/442, 519, 617 nm
346/442 nm
402/421 nm
495, 555, 650/519, 565, 668 nm
495/519 nm
496, 546, 565/578 nm
531/554 nm
555/565 nm
556/573 nm
578/603 nm
590/617 nm
650/660 nm
650/668 nm
752/779 nm
Label or Dye:
APC (Allophycocyanin)
Alexa Fluor 350
Alexa Fluor 350, Alexa Fluor 488, Alexa Fluor 594
Alexa Fluor 405
Alexa Fluor 488
Alexa Fluor 488, Alexa Fluor 555, Alexa Fluor 647
Alexa Fluor 532
Alexa Fluor 546
Alexa Fluor 555
Alexa Fluor 568
Alexa Fluor 594
Alexa Fluor 647
Alexa Fluor 750
Biotin-XX
HRP (Horseradish Peroxidase)
R-PE (R-Phycoerythrin)
Unit Size:
Each
This item is not returnable. View return policy

For Research Use Only. All usage must comply with product instructions.

Product Code. 10013402

Brand: Invitrogen™ Z25306

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This item is not returnable. View return policy

For Research Use Only. All usage must comply with product instructions.

Includes

Contains 1 vial of Zenon Alexa Fluor 568 rabbit IgG labeling reagent (250 ÎĽL), and 1 vial of Zenon blocking reagent (rabbit IgG, 250 ÎĽL).

Generate antibody (IgG) conjugates for immunocytochemistry (ICC), immunohistochemistry (IHC), flow cytometry, and cell imaging with the Invitrogen Zenon™ Rabbit IgG Labeling Kits.

Generate antibody (IgG) conjugates for immunocytochemistry (ICC), immunohistochemistry (IHC), flow cytometry, and cell imaging with the Invitrogen Zenon™ Rabbit IgG Labeling Kits. Simplify your laboratory applications and reduce antibody cross-reactivity while also achieving efficient labeling of antibodies in serum, ascites fluid, or hybridoma suspensions.

Achieve fast, versatile, and reliable fluorophore-, biotin-, or enzyme-labeled IgG primary antibodies with the Zenon™ rabbit IgG labeling kits. These kits utilize Alexa Fluor fluorophores, biotin, Pacific Blue, or enzymes such as R-phycoerythrin and allophycocyanin, which are attached to monovalent, affinity purified Fab fragments. The Fab fragments, in turn, are directed against and bind with the Fc portion of IgG primary antibodies. Only a small amount of starting material is required, and the method is optimized for efficient labeling of antibodies in serum, ascites fluid, or hybridoma suspensions. Because the Zenon labeling method is based on immunoselectivity, it does not require the removal of exogenous proteins (such as serum) or amine-containing buffers from the target antibody, simplifying the process.

Zenon labeling technology greatly simplifies the use of multiple mouse-derived antibodies in the same staining protocol. Zenon tricolor labeling kits contain sufficient materials for 10 labeling reactions of each of three different fluorescent colors.

Important features of Zenon labeling technology:
• Labeled antibodies are typically ready to use in 10 minutes
• Requires only 1–20 μg primary antibody
• Simple—no purification required
• Flexible—choose from different fluorophores, biotin, HRP, alkaline phosphatase
• Multiplex with other mouse monoclonal antibodies simultaneously
• Can be used in a variety of applications including ICC, IHC, flow cytometry, and cell imaging.

Advantages of using Zenon antibody labeling kits include:

Cost savings
Zenon antibody labeling kits offer a cost-conscious and reproducible method of tagging as little as 0.4 ÎĽg in 2 ÎĽL of primary antibody, with minimal waste of expensive or difficult-to-obtain antibodies, or excessive washing steps that pose the risk of product loss.

Sensitivity
Label your primary antibodies without compromising their antigen binding affinity: Zenon dye- and enzyme-labeled Fab fragments, which are targeted to the Fc tail, are affinity purified during their preparation to ensure high affinity and selectivity for the Fc portion of the corresponding primary antibody. The procedure for chemical labeling of the Zenon Fab fragments protects their Fc-binding site, resulting in more active labeling reagents.

Speed
No purification procedure is required prior to using Zenon Fab fragments in your laboratory applications. Formation of the Fab-antibody complex occurs in fewer than five minutes, followed by a five-minute blocking step. During this time, almost all the primary antibody in the mixture is labeled with the labeled Fab fragments.

Simplicity
The Fab-antibody complexes display fluorescence or enzymatic activity that is similar in intensity to that of directly labeled primary antibodies. Varying the extent of the antibody labeling is as simple as changing the amount of added Zenon labeling reagent during the reaction. Once the labeling complexes are formed, they can used immediately, without need for antibody purification.

Reliability
The Zenon Fab-antibody complex is stable and allows subsequent or simultaneous labeling of different target cells and tissues with different complexes. After staining, an aldehyde-based fixing step may be used to prevent the transfer of different labels between different primary antibodies, preserving the initial staining pattern.

Customization
We offer custom antibody conjugation services that are efficient and confidential, and we stand by the quality of our work. We are ISO 13485:2000 certified.

TRUSTED_SUSTAINABILITY

Specifications

Product Type Labeling Kit
Quantity 50 Reactions kit
Content And Storage Contains 1 vial of Zenon Alexa Fluor 568 rabbit IgG labeling reagent (250 ÎĽL), and 1 vial of Zenon blocking reagent (rabbit IgG, 250 ÎĽL).

Store in refrigerator (2–8°C) and protect from light.
Excitation/Emission 578/603 nm
Labeling Scale < 1–20 μg
Product Line Zenon™
Label or Dye Alexa Fluor 568
Color Red-Orange
Labeling Target IgG
Species Rabbit
Label Type Alexa Fluor
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Certificates

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5 results found
Lot Number Certificate Type Date Product Code
Lot Number2192263 Certificate TypeCertificate of Analysis Date10/10/2020 Product CodeZ25306
Lot Number2174882 Certificate TypeCertificate of Analysis Date15/01/2020 Product CodeZ25306
Lot Number2080270 Certificate TypeCertificate of Analysis Date31/03/2019 Product CodeZ25306
Lot Number2747455 Certificate TypeCertificate of Analysis Date31/10/2023 Product CodeZ25306
Lot Number2530228 Certificate TypeCertificate of Analysis Date20/10/2022 Product CodeZ25306

For Research Use Only. Not for use in diagnostic procedures.

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