IBA Lifesciences GmbH

IBA Lifesciences GmbH
Products
IBA Lifesciences GmbH BioLock

IBA Lifesciences GmbH BioLock

BioLock is a Biotin blocking solution for lysates and culture supernatants BioLock - Biotin blocking solution; 250 ml

IBA Lifesciences GmbH 10x Buffer R - Regeneration Buffer

IBA Lifesciences GmbH 10x Buffer R - Regeneration Buffer

10x Buffer R is a Strep-Tactin™ regeneration buffer with HABA 100 ML STREP-TAG(R) REGENERATION BUFFER WITH HABA(10x Buffer R) 100mL Store in 4C Fridge

IBA Lifesciences GmbH StrepMAB-Classic

IBA Lifesciences GmbH StrepMAB-Classic

Monoclonal antibody to detect Strep-tag™II and Twin-Strep-tag™ fusion proteins 100 UG STREPMAB-CLASSIC, STREP-TAG II SPECIFICmonoclonal antibody, mouse/IgG1, purified 100µg

IBA Lifesciences GmbH 10x Buffer BXT

IBA Lifesciences GmbH 10x Buffer BXT

IBA Lifesciences GmbH 10x Buffer BXT; Strep-Tactin™XT Elution B. is a ready-to-use buffer for elution of Strep-tagged proteins from Strep-Tactin™ XT. 25ML 10x Buffer BXT; Strep-TactinXT Elution Buffer

IBA Lifesciences GmbH Twin-Strep-tag™ Capture Kit

IBA Lifesciences GmbH Twin-Strep-tag™ Capture Kit

The Twin-Strep-tag™ Capture Kit can be used for reversible capture of Twin-Strep-tag™ fusion proteins for SPR analyses Twin-Strep-tag capture kit, for coating a BiacoreCM5 sensor chip with Strep-Tactin XT

IBA Lifesciences GmbH Strep-Tactin™ Superflow™ High Capacity Resin

IBA Lifesciences GmbH Strep-Tactin™ Superflow™ High Capacity Resin

50% suspension to purify Strep-tag™II or Twin-Strep-tag™ fusion proteins 20 ML Strep-Tactin(R) Superflow(R) high capacity,50% suspension 20mL Store in 4C Fridge

IBA Lifesciences Anhydrotetracycline

IBA Lifesciences Anhydrotetracycline

CAS: 1665-56-1 Molecular Formula: C22H22N2O7 Molecular Weight (g/mol): 426.43 InChI Key: JGRMImmCZBOWCW-LCGGBVFXSA-N Synonym: anhydrotetracycline, 5 A,6-anhydrotetracycline, 4 s-anhydrotetracycline, cxcveerymjzmmm-docrcchosa-n, 4 s,4 AS,12 AS-4-dimethylamino-3,10,11,12 A-tetrahydroxy-6-methyl-1,12-dioxo-1,4,4 A,5,12,12 A-hexahydrotetracene-2-carboxamide PubChem CID: 54675758 25 MG Anhydrotetracycline 25mg Store in -20CFreezer

IBA Lifesciences GmbH Strep-Tactin™ Sepharose™ Column

IBA Lifesciences GmbH Strep-Tactin™ Sepharose™ Column

Strep-Tactin™ Sepharose™ column is a Gravity flow column to purify Strep-tag™II or Twin-Strep-tag™ fusion proteins X5 Gravity flow Strep-Tactin(R) Sepharose column,

IBA Lifesciences GmbH NY-ESO-1 peptide SLLMWITQV (HLA-A*0201)

IBA Lifesciences GmbH NY-ESO-1 peptide SLLMWITQV (HLA-A*0201)

Single peptide synthesized as presented by the MHC class I allele 1MG NY-ESO-1 peptide SLLMWITQV (HLA-A*0201)

IBA Lifesciences GmbH 10x Buffer W - Wash Buffer

IBA Lifesciences GmbH 10x Buffer W - Wash Buffer

Strep-Tactin™ and Strep-Tactin™XT wash buffer 100 ML Strep-tag(R) washing buffer (10x buffer W)

IBA Lifesciences GmbH Desthiobiotin

IBA Lifesciences GmbH Desthiobiotin

Desthiobiotin powder for preparation of Strep-Tactin™ elution buffer 0.107 GR D-DESTHIOBIOTIN, 0.107G FOR 200ML 1Xbuffer 00 Store in 4C Fridge

IBA Lifesciences GmbH pDSG-IBA Small Mammalian Expression Vector

IBA Lifesciences GmbH pDSG-IBA Small Mammalian Expression Vector

Designed for protein expression for mammalian HEK293E cells (MEXi 293E cells). Small Mammalian Expression Vector is a small transient expression vector especially developed for the use in combination with the MEXi mammalian expression system. pDSG-IBA103; 5µg

IBA Lifesciences GmbH Strep-tag™ Protein Purification Buffer Set, IBA Lifesciences GmbH

IBA Lifesciences GmbH Strep-tag™ Protein Purification Buffer Set, IBA Lifesciences GmbH

Strep-Tactin™ purification buffer set 100ml Strep-Tactin purification buffer set(100mL 10x buffer W, 25mL 10x buffer E, 100mL

IBA Lifesciences GmbH Gravity Flow Strep-Tactin Sepharose 4FF

IBA Lifesciences GmbH Gravity Flow Strep-Tactin Sepharose 4FF

Column, cartridge and plate formats for Strep-Tactin™ resins 10 ML GRAVITY FLOW STREP-TACTIN(R) SEPHAROSEcolumn, 10 mL 00 Store in 4C Fridge

IBA Lifesciences GmbH CMV pp65 peptide NLVPMVATV (HLA-A*0201)

IBA Lifesciences GmbH CMV pp65 peptide NLVPMVATV (HLA-A*0201)

Single peptide synthesized as presented by the MHC class I allele 1MG CMV pp65 peptide NLVPMVATV (HLA-A*0201)

IBA Lifesciences GmbH Ovalbumin peptide SIINFEKL (H-2 Kb)

IBA Lifesciences GmbH Ovalbumin peptide SIINFEKL (H-2 Kb)

Single peptide synthesized as presented by the MHC class I allele 1MG Ovalbumin peptide SIINFEKL (H-2 Kb)

IBA Lifesciences GmbH MART-1 peptide ELAGIGILTV (HLA-A*0201)

IBA Lifesciences GmbH MART-1 peptide ELAGIGILTV (HLA-A*0201)

Single peptide synthesized as presented by the MHC class I allele 1MG MART-1 peptide ELAGIGILTV (HLA-A*0201)

IBA Lifesciences Strep-Tactin™ Conjugate

IBA Lifesciences Strep-Tactin™ Conjugate

Strep-Tactin™ conjugates for detection of Strep-tag™II and Twin-Strep-tag™ fusion proteins 500 UL Strep-Tactin(R) AP conjugate 0.5mL Store

IBA Lifesciences GmbH MHC I-Strep HLA-A*0201; CMV pp65 (NLVPMVATV)

IBA Lifesciences GmbH MHC I-Strep HLA-A*0201; CMV pp65 (NLVPMVATV)

MHC class I molecule fused to a Twin-Strep-tag™ and refolded with a peptide MHC I-Strep HLA-A*0201, CMV pp65, NLVPMVATV; 40 µl

IBA Lifesciences GmbH Biotin Blocking Buffer

IBA Lifesciences GmbH Biotin Blocking Buffer

Biotin Blocking Buffer is optimized for efficient blocking of biotinylated proteins in western blot analyses 2 ML BIOTIN BLOCKING BUFFER 2ML STORE IN 4C FRIDGE

IBA Lifesciences GmbH WET FRED Set

IBA Lifesciences GmbH WET FRED Set

The WET FRED Set is an application aid for large volumes of lysates or culture supernatants WET FRED 1ml Adapter for 1ml Gravitiy flow columns

IBA Lifesciences GmbH pCSG-IBA Mammalian Expression Vector with Secretion

IBA Lifesciences GmbH pCSG-IBA Mammalian Expression Vector with Secretion

Designed for high-level episomal expression in mammalian hosts under G418 selection. Mammalian Expression Vector with Secretion is a large expression vector with universal features for transient expression as well as for generation of stable cell lines (NeoR). pCSG-IBA144; 5µg

IBA Lifesciences Strep-Tactin™ PE or APC

IBA Lifesciences Strep-Tactin™ PE or APC

Fluorophore-conjugated Strep-Tactin™ for cell staining Strep-Tactin PE for MHC I Streptamers; 50 µl

IBA Lifesciences Strep-Tactin™ Superflow™ High Capacity Column

IBA Lifesciences Strep-Tactin™ Superflow™ High Capacity Column

Gravity flow column to purify Strep-tag™II or Twin-Strep-tag™ fusion proteins 1 ML Strep-Tactin(R) Superflow(R) high capacity

IBA Lifesciences GmbH pCSG-IBA Mammalian Expression Vector with No Secretion

IBA Lifesciences GmbH pCSG-IBA Mammalian Expression Vector with No Secretion

Designed for high-level episomal expression in mammalian hosts under G418 selection. Mammalian Expression Vector with No Secretion is a large expression vector with universal features for transient expression as well as for generation of stable cell lines (NeoR). pCSG-IBA64; 5µg

IBA Lifesciences GmbH CD4 Fab-TACS™ Agarose Column Starter Kit

IBA Lifesciences GmbH CD4 Fab-TACS™ Agarose Column Starter Kit

Label-free isolation of CD4+ T cells Label-free isolation of CD4+ T cells from murine splenocytes

IBA Lifesciences GmbH CD8/CD62L/CD45RA Fab-TACS™ Magnetic Microbead Starter Kit

IBA Lifesciences GmbH CD8/CD62L/CD45RA Fab-TACS™ Magnetic Microbead Starter Kit

Label-free isolation of naive or central memory T cells from PBMCs Label-free isolation of naive or central memory Tcells from PBMCs

IBA Lifesciences Strep-Tactin™ 4Flow™ High Capacity Cartridge

IBA Lifesciences Strep-Tactin™ 4Flow™ High Capacity Cartridge

Strep-Tactin™ 4Flow™ high capacity cartridge is a HPLC/FPLC cartridge to purify Strep-tag™II or Twin-Strep-tag™ fusion proteins Strep-TactinXT 4Flow high capacity cartridge, 1 ml

IBA Lifesciences Biotin

IBA Lifesciences Biotin

5 GR D-Biotin (not for Strep-tag applications) 5g

IBA Lifesciences Streptavidin

IBA Lifesciences Streptavidin

Streptavidin is a widely used tool for immobilization and assays of biotinylated proteins or other biotin conjugated molecules. 100 MG Streptavidin (not for Strep-tag applications) 100mg Store in -20C Freezer

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Featured Products

Immobilisation with Near Covalent Binding Affinity


Immobilization with near covalent binding affinity

Twin-Strep-tag™ Capture Kit for SPR Experiments

  • Indirect immobilisation of the ligand prevents the ligand to be inactivated or influenced by the immobilisation procedure
  • High affinity in low pM range enables long term measurements (T1/2 = 13 h)
  • Chip can be easily regenerated by an established procedure
  • Ligands can be captured directly from complex samples due to the high affinity and specificity of the Twin-Strep-tag:Strep-Tactin XT interaction

Try our New High Affinity Strep-tag™ Technology


Try our new high affinity Strep-tag® system

Strep-Tactin XT 4Flow™ Starter Kit consists of:

  • Strep-Tactin XT 4Flow gravity flow column 
  • Buffer W (Strep-Tactin/Strep-Tactin XT Wash Buffer) 
  • Buffer BXT (Strep-Tactin XT Elution Buffer with Biotin) 
  • Buffer XT-R (Strep-Tactin XT Regeneration Buffer with Biotin MgCl2)
  • Strep-Tactin conjugated with HRP (for Western blot detection)
  • Short protocol

Exosome Isolation - Quick and Simple


Try our new high affinity Strep-tag® system

The Fab-TACS™ Exosome Isolation Technology offers:

  • Highly specific and reproducible isolations with minimal contamination in a source-independent manner
  • Quick and simple protocol, without magnetic beads or ultracentrifugation, no extra device needed
  • Label-free exosomes with intact biological functions due to reversible reagents
Cell
IBS's Cell Selection

Traceless Affinity Cell Selection


Traceless Affinity Cell Selection

Fab-TACS™ technology perfectly combines the benefits of positive and negative cell selection

  • High purity, yield and viability
  • Label-free, non-activated cells
  • From whole blood, buffy coat, PBMCs or murine samples
  • Manual or automated cell separation

Magnetic Cell Selection


Magnetic Cell Selection

IBA's Streptamer™

  • Fully reversible
  • No stimulation of cells after cell separation
  • Optimal preservation of cell effector function 
  • Improved viability of cells 

Cell Stimulation & Expansion


Cell Stimulation & Expansion

CD3/CD28 Streptamers™

  • Completely reversible reagents
  • Termination of stimulation at any given point in time
  • Adjustable CD3 : CD28 ratio 
  • Bead-free

Peptides for T cell stimulation

  • Purity > 85 %
  • Endotoxin free

Fluorescent Cell Staining


Fluorescent Cell Staining

Detection of antigen-specific T cells with MHC I Streptamers™ and of receptor-specific T cells with Fab Streptamers™

  • Fully reversible
  • High specificity, sensitivity and reproducibility
  • No leftover of staining reagents on the cells after FACS sorting
  • Optimal preservation of cell effector function
  • Improved viability of cells
Protein
Protein

Cloning


Cloning & Transfection

More than 150 Twin/Strep-tag™ II expression vectors

  • Variety of Twin/Strep-tag II combined with His, FLAG or GST
  • Different N-/C-terminal orientations
  • Expression hosts: E. coli, mammalia, insect cells, yeast
  • Easy cloning via StarGate vectors

Mammalian Expression


Cloning & Transfection

The MEXi mammalian expression system

Optimised for efficient protein expression and Strep-tag purification in mammalian cells.

  • Perfectly matched HEK293E cell line and cell culture media
  • Transient expression for time and cost efficient protein production
  • Multiple expression vectors from IBA with different features

Protein Purification


Protein Purification

Strep-tag™ technology from the original manufacturer

  • High affinity in low pM range
  • Rapid one-step purification with unparalleled protein purity (> 95 %)
  • Mild and specific elution
  • Tolerates broad range of buffer conditions
  • Suitable for metalloproteins, membrane proteins, low abundant proteins, protein complexes and any other

Detection


Detection

For Western blot analysis, immunofluorescence, FACS and ELISA

Two different detection systems are provided:

  • Strep-Tactin and Strep-Tactin XT conjugate for Twin/Strep-tag II proteins
  • Monoclonal antibodies against Twin/Strep-tag II

Immobilisation


Immobilisation & Immunoprecipitation

Near covalent binding affinity – Immobilisation by Strep-Tactin XT: Twin-Strep-tag for screenings, diagnostic assays, protein biochemistry, protein kinetics and interaction studies, SPR experiments (Biacore) or BLI

  • High affinity in low pM range enables measurements over a long time
  • Minimal non-specific binding 
  • Minimal coefficients of variation (cv)
  • Chip for SPR experiments can be easily regenerated
Exosomes

Traceless Affinity Exosome Isolation


Backbone Modifications

Quick and simple isolation of unlabelled exosomes using Fab-TACS™ technology

The Fab-TACS technology for exosome isolation offers

  • Highly specific and reproducible isolations
  • Minimal contamination by other EVs
  • Label-free exosomes with intact biological functions
  • Source-independent application
  • Reversible capture and release of exosomes
  • A quick and simple protocol, without magnetic beads or ultracentrifugation
Resources
IBA_about_us

About IBA Lifesciences GmbH

Solutions For Life Sciences

IBA Lifesciences GmbH provides innovative technologies for life science applications. The product portfolio includes tools for cell selection and expansion as well as protein production and assays.