IBA Lifesciences
IBA Lifesciences Strep-Tactin™ protein as lyophilized powder
Strep-Tactin™ protein as lyophilized powder can be used for reversible immobilization of biotinylated analytes or Strep-tag™II and Twin-Strep-tag™ fusion proteins
IBA Lifesciences 10x Buffer R - Regeneration Buffer
10x Buffer R is a Strep-Tactin™ regeneration buffer with HABA
Promotions are available
IBA Lifesciences Strep-tag™ Protein Purification Buffer Set, IBA Lifesciences GmbH
Strep-Tactin™ purification buffer set
IBA Lifesciences Magnetic Separator
The Magnetic Separator is a device for separation of magnetic beads from residual solution
IBA Lifesciences pASK-IBA E. coli vector
pASK-IBA E. coli expression vectors encoding a Strep-tag™II and chloramphenicol resistance
IBA Lifesciences Strep-tag™II Peptide
Strep-tag™II peptide is a synthetic peptide recognized by Strep-Tactin™ and Strep-Tactin™XT
IBA Lifesciences MEXi-CM
IBA Lifesciences GmbH MEXi-CM Culture Medium is an efficient and cost effective mammalian protein expression system.
IBA Lifesciences MEXi-TM
IBA Lifesciences GmbH MEXi-TM Transfection Medium is an efficient and cost effective mammalian protein expression system.
IBA Lifesciences MHC I-Strep HLA-A*2402; CMV pp65 (QYDPVAALF)
MHC class I molecule fused to a Twin-Strep-tag™ and refolded with a peptide
IBA Lifesciences WET FRED Set
The WET FRED Set is an application aid for large volumes of lysates or culture supernatants
IBA Lifesciences StrepMan Magnet
IBA Lifesciences GmbH StrepMan Magnet is designed to be used for manual cell isolation with Strep-Tactin™ Magnetic Microbeads using the Streptamer™ Technology.
IBA Lifesciences WT1 peptide RMFPNAPYL (HLA-A*0201)
Single peptide synthesized as presented by the MHC class I allele
IBA Lifesciences HA-1H peptide VLHDDLLEA (HLA-A*0201)
Single peptide synthesized as presented by the MHC class I allele
IBA Lifesciences GFP-Strep-tag control protein
GFP-Strep-tag control protein for Strep-Tactin™ and Strep-Tactin™XT applications
IBA Lifesciences StarGate pESG-IBA Vectors for Mammalian Expression
Designed for high-level constitutive expression of recombinant proteins in a wide range of Mammalian host cells through the CMV promoter, with replication in cell lines that are latently infected with SV40 large T antigen.
IBA Lifesciences StarGate pLSG-IBA Vectors for Baculo
Transfer vectors that allow for high-level expression in insect cells by introducing the gene of interest into the Polyhedrin gene locus of AcMNPV DNA by homologous recombination.
IBA Lifesciences MHC I-Strep HLA-A*0201; WT1 (RMFPNAPYL)
MHC class I molecule fused to a Twin-Strep-tag™ and refolded with a peptide
IBA Lifesciences MagStrep 'Type3' XT Beads
MagStrep 'type3' XT beads are magnetic beads (5% suspension) to purify Strep-tag™II or Twin-Strep-tag™ fusion proteins
IBA Lifesciences Strep-Tactin™ Magnetic Microbeads
IBA Lifesciences GmbH Strep-Tactin™ Magnetic Microbeads enable a rapid and economic cell isolation.
IBA Lifesciences Strep-Tactin™ 4Flow™ Cartridge
Strep-Tactin™ 4Flow™ cartridge is a HPLC/FPLC cartridge to purify Strep-tag™II or Twin-Strep-tag™ fusion proteins
IBA Lifesciences Strep-Tactin™ Superflow™ High Capacity Cartridge
Strep-Tactin™ Superflow™ high capacity cartridge is a HPLC/FPLC cartridge to purify Strep-tag™II or Twin-Strep-tag™ fusion proteins
IBA Lifesciences CD8 Fab-TACS™ Agarose Column Starter Kit
Label-free isolation of CD8+ T cells
IBA Lifesciences MHC I-Strep HLA-A 1101; SARS-CoV-2 (RLFRKSNLK); Fused to Twin-Strep-tag
A MHC class I molecule fused to a Twin-Strep-tag™ and refolded with a peptide.
IBA Lifesciences Strep-Tactin™ 4Flow™ High Capacity Cartridge
Strep-Tactin™ 4Flow™ high capacity cartridge is a HPLC/FPLC cartridge to purify Strep-tag™II or Twin-Strep-tag™ fusion proteins
IBA Lifesciences CD45RA Fab-Strep
Low affinity Fab fragment fused to a Twin-Strep-tag™ and specific for human CD45RA
IBA Lifesciences™ MHC I-Strep HLA-A*0201; HER-2/neu (RLLQETELV); Fused to Twin-Strep-tag™
MHC Class I molecule fused to a streptavidin derivative and refolded with a peptide.
IBA Lifesciences™ MHC I-Strep HLA-A*0201; Prame (VLDGLDVLL); Fused to Twin-Strep-tag™
MHC Class I molecule fused to a streptavidin derivative and refolded with a peptide.
IBA Lifesciences™ MHC I-Strep H-2 Db; gp33 LCMV (KAVYNFATM); Fused to Twin-Strep-tag™
MHC Class I molecule fused to a streptavidin derivative and refolded with a peptide.
IBA Lifesciences™ MHC I-Strep HLA-A*0201; HCV NS3 (KLVALGINAV); Fused to Twin-Strep-tag™
MHC Class I molecule fused to a streptavidin derivative and refolded with a peptide.
Immobilization with Near Covalent Binding Affinity
Reversible capture and analysis of Twin-Strep-tag™ fusion proteins for Bio-Layer Interferometry (BLI) or Surface Plasmon Resonance (SPR) experiments
- Indirect immobilization of the ligand prevents it from inactivation or influences of the immobilization procedure
- High specificity and affinity in low pM range of the Twin-Strep-tag™ - Strep-Tactin™ XT interaction
- Ligands can be captured directly from complex samples
- Strep-Tactin™ XT biosensors/chips can be easily regenerated by an established procedure, whereby further ligand-analyte interactions can be analyzed with the same biosensor/chip
Our Perfect Tool for Isolation of Proteins or Complexes
MagStrep 'Type 3' XT Magnetic Beads offer:
- High specificity and low background resulting in high protein purity
- High binding affinity and capacity for Strep-tag™ II or Twin-Strep-tag™ fusion proteins
- Immunoprecipitation (IP) or co-immunoprecipitation (Co-IP) in batch format and/or high throughput screening for protein purification via pull-downs
- Suitable for usage with (semi-)automated magnetic bead purification systems (e.g., Thermo Scientific™ KingFisher™) for automated workflows
Efficient Purification Even of Large Proteins
Strep-Tactin™ XT 4Flow™ Starter Kit consisting of everything you need for a quick start:
- Strep-Tactin™ XT 4Flow™ gravity flow column
- All buffers required for washing, elution and regeneration
- Strep-Tactin™ conjugated with HRP (for Western blot detection)
- Short protocol
Strep-Tactin™ for Reversible Binding of Biotinylated Proteins
Applicable for purification, detection and immobilization of biotinylated molecules
- Suitable for proximity-dependent Biotin Identification (BioID) experiments
- Mild and highly specific elution facilitating analysis of protein complexes
Strep-Tactin™ Sepharose™ and Strep-Tactin™ Superflow™ for gravity flow column-based affinity purification
MagStrep 'Type 3' Strep-Tactin™ magnetic beads are suitable for batch purification as well as pull-down assays
TACS - Traceless Affinity Cell Selection
Fab-TACS™ technology perfectly combines the benefits of positive and negative cell selection
- High purity, yield and viability
- Label-free, non-activated cells due to fully reversible binding
- From whole blood, buffy coat, PBMCs or murine samples
- Affinity chromatographic, magnetic or fluorescent cell separation
MHC I Streptamer™
Detection and isolation of antigen-specific T-cells with MHC I Streptamers™
- Label-free cell isolation according to antigen specificity
- High specificity, sensitivity and reproducibility
- From single cell suspensions (e.g., PBMCs or murine samples)
- Affinity chromatographic, magnetic or fluorescent cell separation
Cell Stimulation & Expansion
- Completely reversible reagents
- Termination of stimulation at any given point in time
- Adjustable CD3: CD28 ratio
- Bead-free
Peptides for T-cell stimulation
- Purity >85%
- Endotoxin-free
Fluorescent Cell Staining
Detection of antigen-specific T-cells with MHC I Streptamers™ and of receptor-specific T-cells with Fab Streptamers™
- Fully reversible
- High specificity, sensitivity and reproducibility
- No leftover staining reagents on the cells after FACS sorting
- Optimal preservation of cell effector function
- Improved viability of cells
Cloning
More than 150 Twin-Strep-tag™/Strep-tag™ II expression vectors
- Variety of Twin-Strep-tag™/Strep-tag™ II combined with His, FLAG or GST
- Different N-/C-terminal orientations
- Expression hosts: E. coli, mammalia, insect cells, yeast
- Easy cloning via StarGate vectors
Mammalian Expression
The MEXi mammalian expression system
Optimised for efficient protein expression and Strep-tag™ purification in mammalian cells.
- Perfectly matched HEK293E cell line and cell culture media
- Transient expression for time and cost efficient protein production
- Multiple expression vectors from IBA with different features
Protein Purification
Strep-tag™ technology from the original manufacturer
- High affinity in low pM range
- Rapid one-step purification with unparalleled protein purity (>95%)
- Mild and specific elution
- Tolerates broad range of buffer conditions
- Suitable for metalloproteins, membrane proteins, low abundant proteins, protein complexes and any other
Detection
For Western blot analysis, immunofluorescence, FACS and ELISA
Two different detection systems are provided:
- Strep-Tactin™ and Strep-Tactin™ XT conjugate for Twin-Strep-tag™/Strep-tag™ II proteins
- Monoclonal antibodies against Twin-Strep-tag™/Strep-tag™ II
Immobilisation
Near covalent binding affinity – Immobilisation by Strep-Tactin XT: Twin-Strep-tag for screenings, diagnostic assays, protein biochemistry, protein kinetics and interaction studies, SPR experiments (Biacore) or BLI
- High affinity in low pM range enables measurements over a long time
- Minimal non-specific binding
- Minimal coefficients of variation (cv)
- Chip for SPR experiments can be easily regenerated
Surface-marker Specific Exosome Isolation
Quick and simple isolation of unlabeled exosomes using Fab-TACS™ for exosomes, which offers:
- Highly specific and reproducible isolation
- Minimal contamination by other EVs
- Label-free exosomes with intact biological functions
- Source-independent application
- Reversible capture and release of exosomes
- A quick and simple protocol, without magnetic beads or ultracentrifugation
Cell Selection & Expansion
- Application Note Central memory T-cells isolated and stained with CD8α and CD62L Fab Streptamer reagents
- Application Note Combining MHC-I and CD8+ Fab Streptamer reagents
- Application Note Regulatory T-cells isolated and stained with CD4-CD25 and CD45RA Fab Streptamer reagents
- Application Note CD3-T Lymphocytes isolated and stained with Streptamer reagents
- Application Note Negative Cell Selection Using Affinity Chromatography
- Application Note Depletion Of Cell Populations From Different Samples
- White Paper Understanding The World Of Mhc I Multimers
Exosomes
Protein Production & Assay
- Application Note Strep Tactin XT vs Strep Tactin high capacity
- Application Note Strep Tactin high capacity
- Application Note Strep Tactin XT Assay
- Application Note Strep Tactin vs Strep Tactin XT
- Application Note Twin Strep tag vs His tag
- Application Note Specific Elution and Avoidance Of Contaminations In Bioid
- Application Note The Strep-Tactin Xt Technology In Downstream Applications
- White Paper Comprehensive Comparison Of The Strep-Tag Technology With The His-Tag System
- White Paper Transient Expression In Mammalian Cells
About IBA Lifesciences
IBA Lifesciences provides high performance research tools for cell and protein isolation based on their proprietary Strep-tag™ technology. The product portfolio offers solutions for the entire production chain of recombinant proteins, from cloning, transfection, protein expression and purification to detection, immobilization and assays, as well as innovative cell isolation and expansion tools.