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IBA Lifesciences

IBA Lifesciences provides high performance research tools for cell and protein isolation based on their proprietary Strep-tag™ technology. Their product portfolio offers solutions for the entire production chain of recombinant proteins, from cloning, transfection, protein expression and purification to detection, immobilization and assays, as well as innovative cell isolation and expansion tools.

 

IBA's Cell Selection and Expansion

TACS - Traceless Affinity Cell Selection

Fab-TACS™ technology perfectly combines the benefits of positive and negative cell selection:

  • High purity, yield and viability
  • Label-free, non-activated cells due to fully reversible binding
  • From whole blood, buffy coat, PBMCs or murine samples
  • Affinity chromatographic, magnetic or fluorescent cell separation
TACS - Traceless Affinity Cell Selection

MHC I Streptamer™

Detection and isolation of antigen-specific T-cells with MHC I Streptamers™:

  • Label-free cell isolation according to antigen specificity
  • High specificity, sensitivity, and reproducibility
  • From single cell suspensions (e.g., PBMCs or murine samples)
  • Affinity chromatographic, magnetic or fluorescent cell separation
MHC I Streptamer™

Cell Stimulation and Expansion

CD3: CD28 Streptamers™:

  • Completely reversible reagents
  • Termination of stimulation at any given point in time
  • Adjustable CD3: CD28 ratio 
  • Bead-free

Peptides for T-Cell Stimulation:

  • Purity >85%
  • Endotoxin-free
Cell Stimulation & Expansion

Fluorescent Cell Staining

Detection of antigen-specific T-cells with MHC I Streptamers™ and of receptor-specific T-cells with Fab Streptamers™:

  • Fully reversible
  • High specificity, sensitivity, and reproducibility
  • No leftover staining reagents on the cells after FACS sorting
  • Optimal preservation of cell effector function
  • Improved viability of cells
Fluorescent Cell Staining

Protein Production and Assay

Cloning

More than 150 Twin-Strep-tag™/Strep-tag™II expression vectors:

  • Variety of Twin-Strep-tag™/Strep-tag™ II combined with His, FLAG or GST
  • Different N-/C-terminal orientations
  • Expression hosts: E. coli, mammalia, insect cells, yeast
  • Easy cloning via StarGate vectors
Cloning

Mammalian Expression

The MEXi mammalian expression system is optimised for efficient protein expression and Strep-tag™ purification in mammalian cells:

  • Perfectly matched HEK293E cell line and cell culture media
  • Transient expression for time and cost efficient protein production
  • Multiple expression vectors with different features
Mammalian Expression

Protein Purification

Strep-tag™ technology from the original manufacturer:

  • High affinity in low pM range
  • Rapid one-step purification with unparalleled protein purity (>95%)
  • Mild and specific elution
  • Tolerates broad range of buffer conditions
  • Suitable for metalloproteins, membrane proteins, low abundant proteins, and protein complexes amongst others
Protein Purification

Detection

For Western blot analysis, immunofluorescence, FACS and ELISA.

Two different detection systems are provided:

  • Strep-Tactin™ and Strep-Tactin™ XT conjugate for Twin-Strep-tag™/Strep-tag™ II proteins
  • Monoclonal antibodies against Twin-Strep-tag™/Strep-tag™ II
Detection

Immobilization

With near covalent binding affinity, – Immobilisation by Strep-Tactin XT: Twin-Strep-tag can be used for screenings, diagnostic assays, protein biochemistry, protein kinetics and interaction studies, surface plasmon resonance (SPR) experiments (Biacore) or bio-layer interferometry (BLI):

  • High affinity in low pM range enables measurements over a long time
  • Minimal non-specific binding 
  • Minimal coefficients of variation (cv)
  • Chip for SPR experiments can be easily regenerated
Immobilisation

Surface-Marker Specific Exosome Isolation

Quick and simple isolation of unlabeled exosomes using Fab-TACS™ for exosomes, which offers:

  • Highly specific and reproducible isolation
  • Minimal contamination by other EVs
  • Label-free exosomes with intact biological functions
  • Source-independent application
  • Reversible capture and release of exosomes
  • A quick and simple protocol, without magnetic beads or ultracentrifugation
Surface-marker Specific Exosome Isolation

Immobilization with Near Covalent Binding Affinity

Reversible capture and analysis of Twin-Strep-tag™ fusion proteins for bio-layer interferometry (BLI) or surface plasmon resonance (SPR) experiments:

  • Indirect immobilization of the ligand prevents it from being subject to inactivation or influences of the immobilization procedure
  • High specificity and affinity in low pM range of the Twin-Strep-tag™ - Strep-Tactin™ XT interaction
  • Ligands can be captured directly from complex samples
  • Strep-Tactin™ XT biosensors/chips can be easily regenerated by an established procedure, whereby further ligand-analyte interactions can be analyzed with the same biosensor/chip
Immobilization with Near Covalent Binding Affinity

Our Perfect Tool for Isolation of Proteins or Complexes

MagStrep 'Type 3' XT Magnetic Beads offer:

  • High specificity and low background resulting in high protein purity
  • High binding affinity and capacity for Strep-tag™ II or Twin-Strep-tag™ fusion proteins
  • Immunoprecipitation (IP) or co-immunoprecipitation (Co-IP) in batch format and/or high throughput screening for protein purification via pull-downs
  • Suitable for usage with (semi-)automated magnetic bead purification systems (e.g., Thermo Scientific™ KingFisher™) for automated workflows
Our Perfect Tool for Isolation of Proteins or Complexes

Efficient Purification Even of Large Proteins

The Strep-Tactin™ XT 4Flow™ Starter Kit consists of everything you need for a quick start:

  • Strep-Tactin™ XT 4Flow™ gravity flow column
  • All buffers required for washing, elution, and regeneration
  • Strep-Tactin™ conjugated with horseradish peroxidase (HRP; for Western blot detection)
  • Short protocol
Efficient Purification Even of Large Proteins

Strep-Tactin™ for Reversible Binding of Biotinylated Proteins

Applicable for purification, detection, and immobilization of biotinylated molecules:

Strep-Tactin™ for Reversible Binding of Biotinylated Proteins

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1 – 15 av 482 Resultat
1

IBA Lifesciences Destiobiotin
GREENER_CHOICE

Destiobiotinpulver för beredning av Strep-Tactin™ elueringsbuffert

EDGE
Kemiskt namn eller material Destiobiotin
För användning med (applikation) För eluering av Strep-Tag II-proteiner från Strep-Tactin
2
Kampanjer är tillgängliga

IBA Lifesciences Strep-™Tactin XT 4Flow™ Snurrkolumnsats med hög kapacitet

Strep-™Tactin XT 4Flow™ hög kapacitet Spin Column Kit för snabb rening av Strep-tag ™II och Twin-Strep-tag™ fusionsproteiner.

EDGE
3

IBA Lifesciences Strep-Tactin ™XT 4Flow™ Harts
BIOPHARMA

Strep-Tactin ™XT 4Flow™ harts är en 50 % suspension för att rena Strep-™tag II eller Twin-Strep-tag™ fusionsproteiner

EDGE
4

IBA Lifesciences Biotin
GREENER_CHOICE

EDGE
5

IBA Lifesciences pASK-IBA E. coli vektor
GREENER_CHOICE

pASK-IBA E. coli-expressionsvektorer som kodar för en Strep-™tag II och kloramfenikolresistens

EDGE
Formatera Suspension
Storleksintervall ca. 3 kb
Replikeringsursprung F1 och ColE1 ursprung
Uttryckssystem E. coli
Antibiotikaresistens bakterie Kloramfenikol
Promotor Tet
Koncentration 250 ng/μ L
Protein Tag Strep-tag II
Kloningsmetod Direkt kloning
Förvaringsbuffert TE-buffert
6

IBA Lifesciences Tyngdkraftsflöde Strep-Tactin™ 4Flow™ hög kapacitet kolumn

Strep-Tactin™ 4Flow™ högkapacitet gravitationsflödeskolonn för att rena Strep-tag ™II eller Twin-Strep-tag™ fusionsproteiner

EDGE
Formatera Gravity flow kolumn
Partikelstorlek 50 till 150μ m
Mål Proteiner märkta med Twin-Strep-tag™ ; Strep-™tag II; korsreaktivitet: biotin
Form Hartsuppslamning
Proteinform Tetrameriskt protein
Kolumntyp Förpackad gravitationsflödeskolonn
Beskrivning 4% agaros, tvärbunden
Slutlig produkttyp Affinitetskromatografiutrustning
Stationär fas Agaros
Bindande kapacitet 20 mg/ml harts
Ligandtyp Twin-Strep-tagg™ ; Strep-™tag II; korsreaktivitet: biotin
7

IBA Lifesciences Strep-Tactin™ 4Flow™ hög kapacitet 50% fjädring

Strep-Tactin™ 4Flow™ hög kapacitet 50% Suspension för att rena Strep-™tag II eller Twin-Strep-tag™ fusionsproteiner

Formatera Flaska
Partikelstorlek 50 till 150μ m
Mål Proteiner märkta med Twin-Strep-tag™ ; Strep-™tag II; korsreaktivitet: biotin
Form Hartsuppslamning
Proteinform Tetrameriskt protein
Kolumntyp Suspension
Beskrivning 4% agaros, tvärbunden
Slutlig produkttyp Affinitetskromatografiutrustning
Typ Bulk harts
Stationär fas Agaros
Koncentration 50% suspension
Bindande kapacitet 20 mg/ml harts
Ligandtyp Twin-Strep-tagg™ ; Strep-™tag II; korsreaktivitet: biotin
8

IBA Lifesciences 10x buffert BXT

IBA Lifesciences GmbH 10x buffert BXT; Strep-Tactin ™XT Elution B. är en färdig att använda buffert för eluering av Strep-märkta proteiner från Strep-Tactin™ XT.

9

IBA Lifesciences MagStrep Strep-Tactin™ XT Beads
GREENER_CHOICE

MagStrep Strep-Tactin™ XT-pärlor är magnetiska pärlor (5 % suspension) för att rena Strep-tag™ II eller Twin-Strep-tag™ fusionsproteiner.

Partikelstorlek 20 till 40μ m
Mål Twin-Strep-tag eller Strep-tag II
Form Hartsuppslamning
Formulering 5 % (v/v) suspension i PBS med 0,1 % (v/v) natriumazid
Typ Magnetisk agarospärla
Typ av stöd 6 % agaros, tvärbunden
10

IBA Lifesciences BioLock
GREENER_CHOICE

BioLock är en biotinblockerande lösning för lysat och odlingssupernatanter

Typ Blockeringslösning
11

IBA Lifesciences 10x Buffer W - Tvättbuffert

Strep-Tactin™ och Strep-™Tactin XT tvättbuffert

12

IBA Lifesciences Strep-Tactin ™XT 4Flow™ Toner med hög kapacitet

Strep-™Tactin XT 4Flow™ högkapacitetspatron är en HPLC/FPLC-patron för att rena Strep-tag ™II eller Twin-Strep-tag™ fusionsproteiner

Formatera Förpackad patron
Partikelstorlek 50 till 150μ m
Mål Twin-Strep-Tag™ och Strep-tag ™II
För användning med (applikation) HPLC/FPLC
Typ av stöd 4% agaros, tvärbunden
13

IBA Lifesciences Strep-Tactin ™XT konjugat
GREENER_CHOICE

Strep-Tactin ™XT konjugerar med fluorescerande märkning för att detektera Strep-™tag II och Twin-Strep-tag™ fusionsproteiner

Innehåll och lagring Outspädd: 2 °C till 8 °C, skydda mot ljus; Utspädd: -25 °C till -15 °C
Kapacitet (metrisk) 0.10 mL
Mål Strep-tag ™II och Twin-Strep-tag™
Typ Konjugera
Kvantitet 50μ g
Kit Innehåll Lösning i PBS; 0,01 % NaN3; 1 mg/ml BSA
Koncentration 0.5 mg/mL
14

IBA Lifesciences Strep-Tactin™ protein som lyofiliserat pulver
GREENER_CHOICE

Strep-Tactin™ protein som lyofiliserat pulver kan användas för reversibel immobilisering av biotinylerade analyter eller Strep-tag ™II och Twin-Strep-tag™ fusionsproteiner

Bindande kapacitet >50 nmol/mg
15

IBA Lifesciences GFP-Strep-tag control protein

GFP-Strep-tag control protein for Strep-Tactin™ and Strep-Tactin™XT applications

Inkluderar 500 μg of a 30 kDa protein

Cell Selection and Expansion

Application Note Central memory T-cells isolated and stained with CD8α and CD62L Fab Streptamer reagents
Filename
ApplicationNote-Central-memory-T-cells-isolated-and-stained-with-CD8α-and-CD62L-Fab-Streptamer-reagents.pdf
Size
662 KB
Format
application/pdf
Application Note-Combining-MHC-I-and-CD8+-Fab-Streptamer-reagents
Filename
Application_Note_Combining.pdf
Size
803 KB
Format
application/pdf
Application Note Regulatory T-cells isolated and stained with CD4-CD25 and CD45RA Fab Streptamer reagents
Filename
ApplicationNote-Regulatory-T-cells-isolated-and-stained-with-CD4-CD25-and-CD45RA-Fab-Streptamer-reagents.pdf
Size
737 KB
Format
application/pdf
Application Note CD3-T Lymphocytes isolated and stained with Streptamer reagents
Filename
ApplicationNote-CD3-T-Lymphocytes-isolated-and-stained-with-Streptamer-reagents.pdf
Size
200 KB
Format
application/pdf
Application Note Negative Cell Selection Using Affinity Chromatography
Filename
Application_Note_Negative_cell_selection_using_affinity_chromatography.pdf
Size
1 MB
Format
application/pdf
Application Note Depletion Of Cell Populations From Different Samples
Filename
Application_Note_Depletion_of_cell_populations_from_different_samples.pdf
Size
1 MB
Format
application/pdf
White Paper Understanding The World Of Mhc I Multimers
Filename
White_Paper_Understanding_The_World_of_MHC_I_Multimers.pdf
Size
5 MB
Format
application/pdf

Protein Production and Assay

ApplicationNote-Strep-Tactin-XT-vs-Strep-Tactin-high-capacity

ApplicationNote-Strep-Tactin-XT-vs-Strep-Tactin-High-Capacity

Filename
ApplicationNote-Strep-Tactin-XT-vs-Strep-Tactin-high-capacity.pdf
Size
193 KB
Format
application/pdf
Application Note Strep Tactin high capacity
Filename
ApplicationNote-Strep-Tactin-high-capacity.pdf
Size
100 KB
Format
application/pdf
Application Note Strep Tactin XT Assay
Filename
ApplicationNote-Strep-Tactin-XT-Assay.pdf
Size
261 KB
Format
application/pdf
Application Note Strep Tactin XT vs Strep Tactin high capacity
Filename
ApplicationNote-Strep-Tactin-vs-Strep-Tactin-XT.pdf
Size
917 KB
Format
application/pdf
Application Note Twin Strep tag vs His tag
Filename
ApplicationNote-Twin-Strep-tag-vs-His-tag.pdf
Size
675 KB
Format
application/pdf
Application Note The Strep-Tactin Xt Technology In Downstream Applications
Filename
Application_note_BLISPR_with_Strep-TactinXT.pdf
Size
1 MB
Format
application/pdf
White Paper Comprehensive Comparison Of The Strep-Tag Technology With The His-Tag System
Filename
White_paper_Comparison_protein_purification_systems.pdf
Size
1 MB
Format
application/pdf
White Paper Transient Expression In Mammalian Cells
Filename
White_paper_Transient_expression_in_mammalian_cells.pdf
Size
1 MB
Format
application/pdf

Exosomes

Isolation of Exosomes from Mesenchymal Stem Cells
Filename
Application_note_exosome_isolation_from_MSC.pdf
Size
1 MB
Format
application/pdf
Isolation of Exosomes from Jurkat Cells and Human Serum
Filename
Application_note_exosome_isolation_from_Jurkat_Serum.pdf
Size
782 KB
Format
application/pdf