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Invitrogen™ Retic Lysate IVT™ Kit Product Code.: 10137194

Invitrogen™ Retic Lysate IVT™ Kit

Product Code. p-4918938
Retic Lysate, 60 Reactions, Each
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Product Type:
Retic Lysate
Retic Lysate with Manual
Quantity:
60 Reactions
Unit Size:
Each
This item is not returnable. View return policy

For Research Use Only. All usage must comply with product instructions.

Product Code. 10137194

Brand: Invitrogen™ AM1200

Genomics Powerhouse
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This item is not returnable. View return policy

For Research Use Only. All usage must comply with product instructions.

Includes

Retic Lysate should be stored at -80°C. 20X Translation Mix minus-Met, 20X Translation Mix minus-Leu, High Salt Mix minus-Met, Low Salt Mix minus-Met, and Capped Xef-1 RNA should be stored below -70°C. Nuclease-free Water can be stored at any temperature.

Efficiently translates high molecular weight proteins and both capped and uncapped mRNAs

The Retic Lysate IVT™ Kit efficiently translates in vitro-synthesized transcripts, poly(A) RNA, and total RNA, including difficult-to-translate RNAs. The optimized lysate results in high protein yield, biological activity, and consistent performance. A report in Nucleic Acids Research (1) found that some commercial reticulocyte lysates had poor fidelity for initiation at the appropriate AUG initiator codon. The Retic Lysate IVT Kit was tested using Kozak's constructs and it demonstrated high fidelity for initiating at the proper AUG.

  • Efficiently translates a wide variety of high molecular weight proteins and both capped and uncapped mRNAs
  • Accommodates large volume of template mRNA
How it works
The lysate has been optimized for translation by the addition of an ATP-regenerating system, hemin, and calf liver tRNA. Included in the kit is an optimized Translation Mix (-Met or -Leu) for the translation of both capped and uncapped messages along with High and Low Salt buffers. These buffers can be used to maximize protein yield and activity from difficult-to-translate sequences.

1. Kozak, M. (1990) Evaluation of the fidelity of initiation of translation in reticulocyte lysates from commercial sources. Nucleic Acids Res 18:2828.

TRUSTED_SUSTAINABILITY

Specifications

Content And Storage • 5 × 200 μl (store at -80°C)
• 2 × 1.75 ml Nuclease-free Water (store at any temperature)
• 100 μl 20X Translation Mix minus-Met
• 100 μl 20X Translation Mix minus-Leu
• 100 μl High Salt Mix minus-Met
• 100 μl Low Salt Mix minus-Met
• 50 μl Capped Xef-1 RNA (50 μg/ml)

Unless otherwise stated, store reagents below -70°C. Do not store cell-free translation systems in the liquid phase of a liquid nitrogen storage system.
Format Kit
Expressed Protein Type Water Soluble Protein
Expression Mechanism Cell-Free Expression
Expression System Cell-Free
For Use With (Application) In Vitro Transcription
No. of Reactions 60 Reactions
Product Line Ambion™
Product Type Retic Lysate
Quantity 60 Reactions
Shipping Condition Dry Ice
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Certificates

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5 results found
Lot Number Certificate Type Date Product Code
Lot Number2821365 Certificate TypeCertificate of Analysis Date29/01/2025 Product CodeAM1200
Lot Number2821373 Certificate TypeCertificate of Analysis Date29/01/2025 Product CodeAM1200
Lot Number2829732 Certificate TypeCertificate of Analysis Date29/01/2025 Product CodeAM1200
Lot Number2821375 Certificate TypeCertificate of Analysis Date29/01/2025 Product CodeAM1200
Lot Number2807682 Certificate TypeCertificate of Analysis Date29/01/2025 Product CodeAM1200

For Research Use Only. Not for use in diagnostic procedures.

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