RiboMinus™ Transcriptome Isolation Kits are novel purification systems that enrich the whole spectrum of RNA transcripts by depleting large ribosomal RNA molecules in a total RNA sample. These kits use a 5μ biotin-labeled, Locked Nucleic Acid (LNA) probe specific for large rRNA to remove 95 to 98% of rRNA molecules from human, mouse, bacteria, or yeast total RNA. This removal increases the representation of RNA transcript species, including non-poly(A) tailed, pre-processed RNA, small tRNAs, siRNA, snRNA, microRNA, fragmented mRNA, and other regulatory RNAs that cannot be purified via poly(A) selection methods.
Purified RNA is concentrated and suitable for numerous downstream applications, including microarray analysis, library construction, and qRT-PCR.
- Comprehensive analysis of the whole transcriptome without contamination or interference from rRNA
- More complete collection of transcribed elements of the genome—no reliance on poly(A) purification methods
Cleaner, clearer microarray analysis due to higher signal-to-background ratios
Not high-throughput compatible (Manual)
DNA and RNA Purification and Analysis, Gene Expression Analysis and Genotyping, Microarray Analysis, RNA Extraction, RNA Isolation for Expression Microarrays, Transcriptome RNA Isolation
|Up to 10μg total RNA|
|Microarray Analysis, Northern Blotting, RT-PCR (Endpoint), Real-Time PCR, cDNA Library Construction|
For Research Use Only. Not for use in diagnostic procedures.