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BL21(DE3)-lysate Rabbit anti-Bacteria, Polyclonal, Proteintech

Rabbit Polyclonal Antibody

Brand:  Proteintech 10558-1-IG-150UL

Additional Details : Weight : 0.00010kg

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Product Code. 16887013

  • 3313.85 SEK / 150µL
Estimated Shipment: 02-12-2021

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Description

Description

Escherichia coli B serves as a research model for studying phage sensitivity, restriction-modification systems, and bacterial evolution, and also as a workhorse for protein expression in life science laboratories and in the biotech industry. Characteristics such as protease deficiency, low acetate production at a high level of glucose, and enhanced permeability (probably due to a simple cell surface) make E. coli B a desirable host for the production of genetically engineered proteins. BL21(DE3) has been very widely used to express recombit proteins. Comparison of BL21(DE3) and another B strain REL606 shows they differ in length by 72. 304 bp and have 426 single nucleotide polymorphisms, all of which have been accounted for by various laboratory manipulations. Differences between B strains and K12 include the absence of flagellar component genes, the DNA cytosine methylase dcm, and ompT in BL21(DE3). B strains may have an additional type II secretion system not found in K12. Other differences include differential distribution of insertions sequences and other changes resulting from horizontal gene transfer. BL21(DE3) also carries a DE3 recombit phage harboring the T7 RNA polymerase gene that can direct high-level expression of cloned genes under the control of the T7 promoter.
Specifications

Specifications

BL21(DE3)-lysate
0.7 mg/mL
PBS with 50% glycerol and 0.1% sodium azide; pH 7.3
BL21(DE3) lysate
BL21(DE3)-lysate Fusion Protein Ag0048
Protein A
RUO
Antibody
Polyclonal
Western Blot
Unconjugated
Liquid
Rabbit
IgG
150 μL
-20°C
Primary
Bacteria
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