Thermo Scientific™ CaptureSelect™ Biotin Anti-C-tag Conjugate

Description

CaptureSelect Biotin Anti-C-tag Conjugate consists of a 13 kDa recombinant single domain antibody fragment (V_HH affinity ligand) with high affinity and selectivity for the 4-amino-acid 'C-tag' peptide tag E‐P‐E‐A (glutamic acid - proline - glutamic acid - alanine). The Biotin Anti-C-tag affinity ligand recognizes the E-P-E-A peptide when this tag is fused directly to the C-terminus of a protein. The affinity ligand, constructed as a mono-specific bihead, is chemically conjugated to biotin via an appropriate spacer that retains the binding reactivity of the ligand when used in combination with streptavidin-based conjugates or streptavidin pre-coated surfaces.

The CaptureSelect Biotin Anti-C-tag Conjugate allows you to

• Detect, quantitate, and characterize any C-tag fusion protein

Applications for CaptureSelect Biotin Anti-C-tag Conjugate include ELISA, Western blot, Gyrolab™-based immunoassays, and label-free detection platforms such as those based on surface plasmon resonance (Biacore™ and IBIS-MX96 systems) and bio-layer interferometry (ForteBio™ Octet™ systems).

Animal origin statement

CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (V_HH) created by a proprietary technology. The V_HH affinity ligand is a 12–15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).