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CD107a (LAMP-1) Mouse anti-Human, PE-Cyanine7, Clone: eBioH4A3, eBioscience™

Mouse Monoclonal Antibody

1136.00 SEK - 2435.00 SEK

Specifications

Antigen CD107a (LAMP-1)
Clone eBioH4A3
Target Species Human
Conjugate PE-Cyanine7
Isotype IgG1, kappa
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Product Code Brand Quantity Price Quantity & Availability  
15568456
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Affymetrix eBioscience
25-1079-41
25 Tests
1136.00 SEK
25 tests
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Estimated Shipment
02-03-2021
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15578456
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Affymetrix eBioscience
25-1079-42
100 Tests
2435.00 SEK
100 tests
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Description

Description

Description: The eBioH4A3 monoclonal antibody reacts with human CD107a, also known as lysosomal-associated membrane protein-1 (LAMP-1). CD107a is a highly glycosylated protein of approximately 110kDa. It is predomitly expressed intracellularly in the lysosomal/endosomal membrane in nearly all cells. CD107a is transiently expressed on the cell surface of degranulating cytolytic T cells, and is also upregulated on the surface of activated platelets and some cancer cells. Applications Reported: This eBioH4A3 antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This eBioH4A3 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of Jurkat cells. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation.

Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 μm post-manufacturing filtered. CD107a (lysosome-associated membrane protein-1, LAMP-1), together with LAMP-2, is a major constituent of lysosomal membrane, 1-2% of total CD107a is found also on the plasma membrane. The LAMP proteins are involved in lysosome biogenesis and are required for fusion of lysosomes with phagosomes. Increased CD107a immunoreactivity is observed in neurones, and in glial cells surrounding senile plaques in Alzheimers disease cases and is localized mainly in medullary epithelial cells, single macrophages and lymphocytes in acute thymic involution. CD107a is a good marker of mast cell activation.
Specifications

Specifications

CD107a (LAMP-1)
Human
IgG1, kappa
Monoclonal
Liquid
Antibody
4° C, store in dark, DO NOT FREEZE!
eBioH4A3
PE-Cyanine7
Flow Cytometry
Primary
Monoclonal
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
RUO
SDS

For Research Use Only.