missing translation for 'onlineSavingsMsg'
Learn More

CD107a (LAMP-1) Rat anti-Mouse, Clone: eBio1D4B (1D4B), eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-1071-82

Additional Details : Weight : 0.01000kg

 View more versions of this product

Product Code. 15227167

  • 1018.00 SEK / 100µg

In stock, ready to ship: 1

Please to purchase this item. Need a web account? Register with us today!


Description

Description

Description: The eBio1D4B monoclonal antibody reacts with mouse CD107a, also known as lysosomal-associated membrane protein-1 (LAMP-1). CD107a is a type I, lysosomal membrane protein that is extensively glycosylated. It is expressed constitutively in the late endosomes-lysosomes in all cells. CD107a is also transiently expressed on the cell surface of degranulating cytolytic T cells. Additionally, CD107a has been implicated in a variety of cellular functions including cancer metastasis and is also a marker for lysosomal storage disorders. Applications Reported: This eBio1D4B (1D4B) antibody has been reported for use in intracellular staining followed by flow cytometric analysis, immunoprecipitation, western blotting, immunohistochemical staining of frozen tissue sections, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections. (Fluorochrome conjugated eBio1D4B (1D4B) is recommended for use in intracellular flow cytometry.). Applications Tested: This eBio1D4B (1D4B) antibody has been tested by intracellular staining and flow cytometric analysis of mouse thioglycolate-elicited peritoneal exudate cells. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered. CD107a (lysosome-associated membrane protein-1, LAMP-1), together with LAMP-2, is a major constituent of lysosomal membrane; 1-2% of total CD107a is found also on the plasma membrane. The LAMP proteins are involved in lysosome biogenesis and are required for fusion of lysosomes with phagosomes. Increased CD107a immunoreactivity is observed in neurons, and in glial cells surrounding senile plaques in Alzheimer′s disease cases and is localized mainly in medullary epithelial cells, single macrophages and lymphocytes in acute thymic involution. CD107a is a good marker of mast cell activation.
Specifications

Specifications

CD107a (LAMP-1)
Monoclonal
0.5 mg/mL
PBS with 0.09% sodium azide; pH 7.2
P11438
CD107a, LAMPA, LGP120, CD107 antigen-like family member A, LAMP-1, lysosome-associated membrane glycoprotein 1, lysosome-associated membrane protein 1
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Mouse
Flow Cytometry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
eBio1D4B (1D4B)
Unconjugated
Lamp1
Liquid
Lamp1
IgG2a, kappa
100 μg
4° C
Primary
16783
Videos
SDS
Documents

For Research Use Only.