CD107b (LAMP-2) Rat anti-Mouse, PE, Clone: M3/84, eBioscience™

Description

Description: The M3/84 monoclonal antibody reacts with mouse Mac-3, the 110 kDa protein expressed by mononuclear phagocytes. The Mac-3 antigen is expressed intracellularly by bone marrow monocyte lineage and is upregulated during macrophage differentiation. Peritoneal and tissue macrophages, dendritic cells, and endothelial cells express this antigen on their surface. It is reported that Mac-3 may be identical to CD107b. Applications Reported: The M3/84 antibody has been reported for use in flow cytometric analysis. Applications Tested: The M3/84 antibody has been tested by flow cytometric analysis of mouse bone marrow and thioglycolate-elicited peritoneal exudates cells and can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 μm post-manufacturing filtered.

Lysosomes are membrane-bound vacuoles which play a critical role in cellular metabolism. These organelles participate in endocytosis, the synthesis and assembly of acid hydrolases, act as sites for digestion of foreign materials and for specialized autolytic cellular processes. Membrane-associated glycoproteins have an important role in normal lysosomal function. Prior to posttranslational modification, Lysosome Associated Membrane Protein 2 (LAMP 2) is an ∽45 kDa polypeptide. Mature, functional LAMP 2 is extensively glycosylated with a variety of different N-linked and O-linked oligosaccharides with a total molecular weight of ∽100-110 kDa. LAMP 2 has a structure comprised of a large amino-terminal intra-lysosomal domain, a hydrophobic trans-membrane domain, and a short carboxyl-terminal cytoplasmic tail.