CD107b (LAMP-2), PE, clone: M3/84, eBioscience™

Description

Description: The M3/84 monoclonal antibody reacts with mouse Mac-3, the 110kDa protein expressed by mononuclear phagocytes. The Mac-3 antigen is expressed intracellularly by bone marrow monocyte lineage and is upregulated during macrophage differentiation. Peritoneal and tissue macrophages, dendritic cells, and endothelial cells express this antigen on their surface. It is reported that Mac-3 may be identical to CD107b.Applications Reported: The M3/84 antibody has been reported for use in flow cytometric analysis.Applications Tested: The M3/84 antibody has been tested by flow cytometric analysis of mouse bone marrow and thioglycolate-elicited peritoneal exudates cells and can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.