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CD117 (c-Kit) Rat anti-Mouse, APC-eFluor™ 780, Clone: ACK2, eBioscience™

Rat Monoclonal Antibody

1048.00 SEK - 1910.00 SEK

Specifications

Antigen CD117 (c-kit)
Clone ACK2
Target Species Mouse
Conjugate APC-eFluor 780
Isotype IgG2b, kappa
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Products 2
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
15371640
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Affymetrix eBioscience
47-1172-80
25 μg
1048.00 SEK
25µg
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Estimated Shipment: 02-03-2022
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15381640
View Documents Promotion Details
Affymetrix eBioscience
47-1172-82
100 μg
1910.00 SEK
100µg
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In stock, ready to ship: 1
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Description

Description

Description: The ACK2 monoclonal antibody reacts with mouse CD117, also known as c-Kit receptor, Steel factor receptor and stem cell factor receptor. A member of the tyrosine kinase receptor family, this 145 kDa molecule is expressed by a majority of hematopoietic progenitor cells characterized in the mouse bone marrow as a small subset of cells positive for Sca-1 and Thy1 (Thy1^lo) and negative for lineage markers. The interaction of the mouse c-kit receptor and steel factor promotes the proliferation and differentiation of hematopoietic progenitor cells. CD117 is also expressed by mast cells and plays a role in signaling and activation of these cells. ACK2 has been reported to be a blocking antibody. Applications Reported: This ACK2 antibody has been reported for use in flow cytometric analysis. Applications Tested: This ACK2 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. APC-eFluor 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Light sensitivity: This tandem is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser. Filtration: 0.2 μm post-manufacturing filtered. c-Kit, also known as CD117 and stem cell factor receptor, is a 145 kDa transmembrane tyrosine kinase encoded by the c-Kit proto-oncogene. c-Kit acts to regulate a variety of biological responses including cell proliferation, apoptosis, chemotaxis and adhesion. Ligand binding to the extracellular domain leads to autophosphorylation on several tyrosine residues within the cytoplasmic domain, and activation. c-Kit mutations correlate with tumor growth and progression in a variety of cancers including mast cell disease, gastrointestinal stromal tumor, acute myeloid leukemia, Ewing sarcoma, and lung cancer. Phosphorylation at tyrosine 703 of c-Kit allows binding of Grb2 and activation of the Ras-Raf-ERK1&2 signaling pathway.
Specifications

Specifications

CD117 (c-kit)
Mouse
IgG2b, kappa
Monoclonal
Liquid
Antibody
4° C, store in dark, DO NOT FREEZE!
ACK2
APC-eFluor 780
Flow Cytometry
Primary
Monoclonal
PBS with 0.09% sodium azide; pH 7.2
RUO
SDS
Product Certifications

For Research Use Only.