CD20, PE, clone: QCH6A7, eBioscience™

Description

Description: The monoclonal antibody QCH6A7 reacts with mouse CD20. CD20 is a B cell specific, 32-kDa membrane protein that is expressed on late pro-B cells through memory B cells in the B cell lineage. CD20 is not found on hematopoietic stem cells, early pro-B cells or later mature plasma cells. CD20 is involved in intracellular calcium regulation and plays a role in B cell proliferation. CD20 is intially expressed after the induction of CD19 during development and is coexpressed with IgM during the pre-B to immature B cell transition in bone marrow. Expression of CD20 increases during maturation with almost all mature B cells expressing some level of CD20. Unlike other B cell antigens, CD20 is not shed or internalized upon antibody binding. Monoclonal antibodies against the CD20 antigen may be used to deplete B cells and CD20 has proven to be an effective immunotheraputic target in B cell lymphomas and several autoimmune diseases including rheumatoid arthritis and type I diabetes. CD20 consists of 4 transmembrane domains and a 44 amino acid extracellular domain which contains the QCH6A7 epitope.Staining with clone QCH6A7 provides significantly improved staining intensity compared to clone AISB12 in flow cytometric assays.Applications Reported: This QCH6A7 antibody has been reported for use in flow cytometric analysis.Applications Tested: This QCH6A7 antibody has been tested by flow cytometric analysis of mouse bone marrow cells and mouse splenocytes. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.