missing translation for 'onlineSavingsMsg'
Learn More

Invitrogen™ CD221 (IGF1R) Monoclonal Antibody (1H7), APC, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Brand:  Invitrogen™ 17-8849-42

 View more versions of this product

Product Code. 15568206

  • 3095.00 SEK / 100 tests

Please to purchase this item. Need a web account? Register with us today!

This item is not returnable. View return policy

Description

Description

Description: This 1H7 monoclonal antibody reacts with human insulin-like growth factor-1 receptor (IGF-1R), also known as CD221. This receptor is a cell surface-expressed glycoprotein composed of two extracellular alpha subunits and two transmembrane beta subunits that possess tyrosine kinase activity. Expressed on nearly all cell types, this receptor binds IGF-I and IGF-II, as well as insulin. Ligand binding leads to activation of the PI3K/Akt and MAPK pathways, which mediate cell proliferation and survival. Many tumors and transformed cells display altered IGF-1R expression. This monoclonal antibody has been reported to block binding of IGF-I and IGF-II to the IGF-1R. Applications Reported: This 1H7 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 1H7 antibody has been pre-titrated and tested by flow cytometric analysis of the HeLa cell line. This can be used at 5 ÎĽL (0.25 ÎĽg) per test. A test is defined as the amount (ÎĽg) of antibody that will stain a cell sample in a final volume of 100 ÎĽL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser. Filtration: 0.2 ÎĽm post-manufacturing filtered.

IGF1R, also known as Insulin-like Growth Factor 1 (IGF-1) Receptor, is a receptor tyrosine kinase activated by IGF-1 and IGF-2. IGFR has been implicated in a variety of cancers. The protein is a tetramer consisting of two alpha, and two beta subunits. The alpha-subunits binds to insulin growth factor 1 with higher affinity than to insulin growth factor 2. Binding results in a conformational change, followed by autophosphorylation of IGF-1R at positions 1134, 1135, 1136. The activated IGF-1R in turn, causes phosphorylation of substrate proteins, followed by sequential activation of RAS, RAF, and mitogen-activated protein kinase isoforms ERK, p38, and JNK, leading to the transcription of genes that drive proliferation.
TRUSTED_SUSTAINABILITY
Specifications
Show More
Product Suggestions

Product Suggestions

Videos
SDS
Documents

Documents

Certificates
Promotions

Promotions

Product Content Correction

Your input is important to us. Please complete this form to provide feedback related to the content on this product.

Product Title
Invitrogen™ CD221 (IGF1R) Monoclonal Antibody (1H7), APC, eBioscience™, Invitrogen™ > 100 Tests; APC

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Thank You! Your feedback has been submitted. Fisher Scientific is always working to improve our content for you. We appreciate your feedback.

For Research Use Only.