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CD25 Rat anti-Mouse, PerCP-Cyanine5.5, Clone: PC61.5, eBioscience™

Rat Monoclonal Antibody

1054.00 SEK - 2600.00 SEK

Specifications

Antigen CD25
Clone PC61.5
Target Species Mouse
Conjugate PerCP-Cyanine5.5
Isotype IgG1, lambda
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Products 2
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
15361150
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Affymetrix eBioscience
45-0251-80
25 μg
1054.00 SEK
25µg
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15231029
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Affymetrix eBioscience
45-0251-82
100 μg
2600.00 SEK
100µg
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In stock, ready to ship: 3
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Description

Description

Description: The PC61.5 antibody reacts with mouse CD25, the 55 kDa interleukin-2 receptor alpha chain (IL-2R alpha). CD25 is expressed by early progenitors of the T and B lineage as well as by activated mature T and B lymphocytes. By itself, CD25 binds IL-2 only with low affinity. However, CD25 associates with CD122 (IL-2 receptor beta chain) and CD132 (common gamma chain) to form the high affinity IL-2 receptor. Binding of IL-2 to both the high and low affinity classes of IL-2 receptor is inhibited by the PC61.5 antibody. CD25 plays a role in lymphocyte differentiation and activation/proliferation. Applications Reported: This PC61.5 antibody has been reported for use in flow cytometric analysis. Applications Tested: This PC61.5 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488 nm; Emission: 695 nm; Laser: Blue Laser. Filtration: 0.2 μm post-manufacturing filtered.

CD25 (IL2 receptor alpha chain/IL2RA) is a cytokine that plays a role in the proliferation of T and B lymphocytes. The receptor of this cytokine (IL2RA) is a heterotrimeric protein complex with a gamma chain also shared by interleukin 4 (IL4) and interleukin 7 (IL7). IL2RA, IL2R beta chain (IL2RB), and the IL2R gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric IL2RA chains result in low-affinity receptor, while homodimeric IL2RB chains produce a medium-affinity receptor. The expression of IL2 in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. IL2 is primarily produced by mature T cells. IL2 plays an important role as a growth factor, differentiation factor, and regulator of cell death. IL-2 stimulates the proliferation of B cells, augments natural killer cell activity, and inhibits granulocyte macrophage colony formation. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests a role in the immune response to antigenic stimuli. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.
Specifications

Specifications

CD25
Mouse
IgG1, lambda
Monoclonal
Liquid
Antibody
4° C, store in dark, DO NOT FREEZE!
PC61.5
PerCP-Cyanine5.5
Flow Cytometry
Primary
Monoclonal
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
RUO
SDS
Product Certifications

For Research Use Only.